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dc.contributor.authorDammeyer, Thorben
dc.contributor.authorSteinwand, Miriam
dc.contributor.authorKrüger, Sarah-C
dc.contributor.authorDübel, Stefan
dc.contributor.authorHust, Michael
dc.contributor.authorTimmis, Kenneth N
dc.date.accessioned2016-01-20T14:30:25Zen
dc.date.available2016-01-20T14:30:25Zen
dc.date.issued2011en
dc.identifier.citationEfficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 2011, 10:11 Microb. Cell Fact.en
dc.identifier.issn1475-2859en
dc.identifier.pmid21338491en
dc.identifier.doi10.1186/1475-2859-10-11en
dc.identifier.urihttp://hdl.handle.net/10033/594409en
dc.description.abstractRecombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.
dc.language.isoenen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshC-Reactive Proteinen
dc.subject.meshHumansen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMucin-1en
dc.subject.meshPeriplasmen
dc.subject.meshProtein Bindingen
dc.subject.meshPseudomonas putidaen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshSingle-Chain Antibodiesen
dc.subject.meshSolubilityen
dc.titleEfficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen
refterms.dateFOA2018-06-13T03:59:17Z
html.description.abstractRecombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.


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