Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.
supplementary figures and table
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Crespo, Carolina Lage
King, Megan C
Alberts, Arthur S
MetadataShow full item record
AbstractCell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells' requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.
CitationPerinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. 2016, 7:10997 Nat Commun
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- The branched actin nucleator Arp2/3 promotes nuclear migrations and cell polarity in the C. elegans zygote.
- Authors: Xiong H, Mohler WA, Soto MC
- Issue date: 2011 Sep 15
- Function and regulation of the Arp2/3 complex during cell migration in diverse environments.
- Authors: Swaney KF, Li R
- Issue date: 2016 Oct
- Exo70 stimulates the Arp2/3 complex for lamellipodia formation and directional cell migration.
- Authors: Liu J, Zhao Y, Sun Y, He B, Yang C, Svitkina T, Goldman YE, Guo W
- Issue date: 2012 Aug 21
- Arp2/3 complex-mediated actin polymerisation occurs on specific pre-existing networks in cells and requires spatial restriction to sustain functional lamellipod extension.
- Authors: Shao D, Forge A, Munro PM, Bailly M
- Issue date: 2006 Jul
- A new mechanism for nuclear import by actin-based propulsion used by a baculovirus nucleocapsid.
- Authors: Au S, Wu W, Zhou L, Theilmann DA, Panté N
- Issue date: 2016 Aug 1