Empowering a mesophilic inoculum for thermophilic nitrification: Growth mode and temperature pattern as critical proliferation factors for archaeal ammonia oxidizers.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsCourtens, Emilie N P
Pieper, Dietmar H
Vlaeminck, Siegfried E
MetadataShow full item record
AbstractCost-efficient biological treatment of warm nitrogenous wastewaters requires the development of thermophilic nitrogen removal processes. Only one thermophilic nitrifying bioreactor was described so far, achieving 200 mg N L(-1) d(-1) after more than 300 days of enrichment from compost samples. From the practical point of view in which existing plants would be upgraded, however, a more time-efficient development strategy based on mesophilic nitrifying sludge is preferred. This study evaluated the adaptive capacities of mesophilic nitrifying sludge for two linear temperature increase patterns (non-oscillating vs. oscillating), two different slopes (0.25 vs. 0.08 °C d(-1)) and two different reactor types (floc vs. biofilm growth). The oscillating temperature pattern (0.25 °C d(-1)) and the moving bed biofilm reactor (0.08 °C d(-1)) could not reach nitrification at temperatures higher than 46 °C. However, nitrification rates up to 800 mg N L(-1) d(-1) and 150 mg N g(-1) volatile suspended solids d(-1) were achieved at a temperature as high as 49 °C by imposing the slowest linear temperature increase to floccular sludge. Microbial community analysis revealed that this successful transition was related with a shift in ammonium oxidizing archaea dominating ammonia oxidizing bacteria, while for nitrite oxidation Nitrospira spp. was constantly more abundant than Nitrobacter spp.. This observation was accompanied with an increase in observed sludge yield and a shift in maximal optimum temperature, determined with ex-situ temperature sensitivity measurements, predicting an upcoming reactor failure at higher temperature. Overall, this study achieved nitrification at 49 °C within 150 days by gradual adaptation of mesophilic sludge, and showed that ex-situ temperature sensitivity screening can be used to monitor and steer the transition process.
CitationEmpowering a mesophilic inoculum for thermophilic nitrification: Growth mode and temperature pattern as critical proliferation factors for archaeal ammonia oxidizers. 2016, 92:94-103 Water Res.
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal.
- Authors: Courtens EN, Spieck E, Vilchez-Vargas R, Bodé S, Boeckx P, Schouten S, Jauregui R, Pieper DH, Vlaeminck SE, Boon N
- Issue date: 2016 Sep
- Change in ammonia-oxidizing microorganisms in enriched nitrifying activated sludge.
- Authors: Sonthiphand P, Limpiyakorn T
- Issue date: 2011 Feb
- Anammox enrichment from reject water on blank biofilm carriers and carriers containing nitrifying biomass: operation of two moving bed biofilm reactors (MBBR).
- Authors: Zekker I, Rikmann E, Tenno T, Lemmiksoo V, Menert A, Loorits L, Vabamäe P, Tomingas M, Tenno T
- Issue date: 2012 Jul
- Nitrifying moving bed biofilm reactor (MBBR) biofilm and biomass response to long term exposure to 1 °C.
- Authors: Hoang V, Delatolla R, Abujamel T, Mottawea W, Gadbois A, Laflamme E, Stintzi A
- Issue date: 2014 Feb 1
- Stable partial nitritation for low-strength wastewater at low temperature in an aerobic granular reactor.
- Authors: Isanta E, Reino C, Carrera J, Pérez J
- Issue date: 2015 Sep 1