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dc.contributor.authorRighetti, Francescoen
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorTwittenhoff, Christianen
dc.contributor.authorBeele, Saschaen
dc.contributor.authorUrban, Kristinaen
dc.contributor.authorWill, Sebastianen
dc.contributor.authorBernhart, Stephan Hen
dc.contributor.authorStadler, Peter Fen
dc.contributor.authorDersch, Petraen
dc.contributor.authorNarberhaus, Franzen
dc.date.accessioned2016-07-05T09:05:34Z
dc.date.available2016-07-05T09:05:34Z
dc.date.issued2016-06-28
dc.identifier.citationTemperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis. 2016, 113 (26):7237-42 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490
dc.identifier.pmid27298343
dc.identifier.doi10.1073/pnas.1523004113
dc.identifier.urihttp://hdl.handle.net/10033/615497
dc.description.abstractRNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 °C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 °C, 37 °C, and 42 °C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTemperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
refterms.dateFOA2018-06-12T22:07:43Z
html.description.abstractRNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 °C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 °C, 37 °C, and 42 °C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.


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