Show simple item record

dc.contributor.authorYakimov, Michail M
dc.contributor.authorCrisafi, Francesca
dc.contributor.authorMessina, Enzo
dc.contributor.authorSmedile, Francesco
dc.contributor.authorLopatina, Anna
dc.contributor.authorDenaro, Renata
dc.contributor.authorPieper, Dietmar H
dc.contributor.authorGolyshin, Peter N
dc.contributor.authorGiuliano, Laura
dc.date.accessioned2016-08-03T13:29:16Z
dc.date.available2016-08-03T13:29:16Z
dc.date.issued2016-08
dc.identifier.citationAnalysis of defence systems and a conjugative IncP-1 plasmid in the marine polyaromatic hydrocarbons-degrading bacterium Cycloclasticus sp. 78-ME. 2016, 8 (4):508-19 Environ Microbiol Repen
dc.identifier.issn1758-2229
dc.identifier.pmid27345842
dc.identifier.doi10.1111/1758-2229.12424
dc.identifier.urihttp://hdl.handle.net/10033/617881
dc.description.abstractMarine prokaryotes have evolved a broad repertoire of defence systems to protect their genomes from lateral gene transfer including innate or acquired immune systems and infection-induced programmed cell suicide and dormancy. Here we report on the analysis of multiple defence systems present in the genome of the strain Cycloclasticus sp. 78-ME isolated from petroleum deposits of the tanker 'Amoco Milford Haven'. Cycloclasticus are ubiquitous bacteria globally important in polyaromatic hydrocarbons degradation in marine environments. Two 'defence islands' were identified in 78-ME genome: the first harbouring CRISPR-Cas with toxin-antitoxin system, while the second was composed by an array of genes for toxin-antitoxin and restriction-modification proteins. Among all identified spacers of CRISPR-Cas system only seven spacers match sequences of phages and plasmids. Furthermore, a conjugative plasmid p7ME01, which belongs to a new IncP-1θ ancestral archetype without any accessory mobile elements was found in 78-ME. Our results provide the context to the co-occurrence of diverse defence mechanisms in the genome of Cycloclasticus sp. 78-ME, which protect the genome of this highly specialized PAH-degrader. This study contributes to the further understanding of complex networks established in petroleum-based microbial communities.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAnalysis of defence systems and a conjugative IncP-1 plasmid in the marine polyaromatic hydrocarbons-degrading bacterium Cycloclasticus sp. 78-ME.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalEnvironmental microbiology reportsen
refterms.dateFOA2017-08-01T00:00:00Z
html.description.abstractMarine prokaryotes have evolved a broad repertoire of defence systems to protect their genomes from lateral gene transfer including innate or acquired immune systems and infection-induced programmed cell suicide and dormancy. Here we report on the analysis of multiple defence systems present in the genome of the strain Cycloclasticus sp. 78-ME isolated from petroleum deposits of the tanker 'Amoco Milford Haven'. Cycloclasticus are ubiquitous bacteria globally important in polyaromatic hydrocarbons degradation in marine environments. Two 'defence islands' were identified in 78-ME genome: the first harbouring CRISPR-Cas with toxin-antitoxin system, while the second was composed by an array of genes for toxin-antitoxin and restriction-modification proteins. Among all identified spacers of CRISPR-Cas system only seven spacers match sequences of phages and plasmids. Furthermore, a conjugative plasmid p7ME01, which belongs to a new IncP-1θ ancestral archetype without any accessory mobile elements was found in 78-ME. Our results provide the context to the co-occurrence of diverse defence mechanisms in the genome of Cycloclasticus sp. 78-ME, which protect the genome of this highly specialized PAH-degrader. This study contributes to the further understanding of complex networks established in petroleum-based microbial communities.


Files in this item

Thumbnail
Name:
Yakimov et al.pdf
Size:
512.4Kb
Format:
PDF
Description:
original manuscript
Thumbnail
Name:
Supporting Information.pdf
Size:
3.115Mb
Format:
PDF
Description:
supporting information
Thumbnail
Name:
Figure 1.tif
Size:
904.4Kb
Format:
TIFF image
Description:
figure 1
Thumbnail
Name:
Figure 4.tif
Size:
4.267Mb
Format:
TIFF image
Description:
figure 4

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by-nc-sa/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/