Subclones in B-lymphoma cell lines: isogenic models for the study of gene regulation.
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MacLeod, Roderick Af
Uphoff, Cord C
Drexler, Hans G
MetadataShow full item record
AbstractGenetic heterogeneity though common in tumors has been rarely documented in cell lines. To examine how often B-lymphoma cell lines are comprised of subclones, we performed immunoglobulin (IG) heavy chain hypermutation analysis. Revealing that subclones are not rare in B-cell lymphoma cell lines, 6/49 IG hypermutated cell lines (12%) consisted of subclones with individual IG mutations. Subclones were also identified in 2/284 leukemia/lymphoma cell lines exhibiting bimodal CD marker expression. We successfully isolated 10 subclones from four cell lines (HG3, SU-DHL-5, TMD-8, U-2932). Whole exome sequencing was performed to molecularly characterize these subclones. We describe in detail the clonal structure of cell line HG3, derived from chronic lymphocytic leukemia. HG3 consists of three subclones each bearing clone-specific aberrations, gene expression and DNA methylation patterns. While donor patient leukemic cells were CD5+, two of three HG3 subclones had independently lost this marker. CD5 on HG3 cells was regulated by epigenetic/transcriptional mechanisms rather than by alternative splicing as reported hitherto. In conclusion, we show that the presence of subclones in cell lines carrying individual mutations and characterized by sets of differentially expressed genes is not uncommon. We show also that these subclones can be useful isogenic models for regulatory and functional studies.
CitationSubclones in B-lymphoma cell lines: isogenic models for the study of gene regulation. 2016: Oncotarget
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig.
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- Somatic diversification and selection of immunoglobulin heavy and light chain variable region genes in IgG+ CD5+ chronic lymphocytic leukemia B cells.
- Authors: Hashimoto S, Dono M, Wakai M, Allen SL, Lichtman SM, Schulman P, Vinciguerra VP, Ferrarini M, Silver J, Chiorazzi N
- Issue date: 1995 Apr 1
- IgVH mutational status and clonality analysis of Richter's transformation: diffuse large B-cell lymphoma and Hodgkin lymphoma in association with B-cell chronic lymphocytic leukemia (B-CLL) represent 2 different pathways of disease evolution.
- Authors: Mao Z, Quintanilla-Martinez L, Raffeld M, Richter M, Krugmann J, Burek C, Hartmann E, Rudiger T, Jaffe ES, Müller-Hermelink HK, Ott G, Fend F, Rosenwald A
- Issue date: 2007 Oct
- Immunoglobulin V gene expression in CD5 B-cell malignancies.
- Authors: Kipps TJ, Rassenti LZ, Duffy S, Johnson T, Kobayashi R, Carson DA
- Issue date: 1992 May 4
- Lack of intraclonal diversification in Ig heavy and light chain V region genes expressed by CD5+IgM+ chronic lymphocytic leukemia B cells: a multiple time point analysis.
- Authors: Schettino EW, Cerutti A, Chiorazzi N, Casali P
- Issue date: 1998 Jan 15
- Common clonal origin of chronic lymphocytic leukemia and high-grade lymphoma of Richter's syndrome.
- Authors: Cherepakhin V, Baird SM, Meisenholder GW, Kipps TJ
- Issue date: 1993 Nov 15