• Brevundimonas vancanneytii sp. nov., isolated from blood of a patient with endocarditis.

      Estrela, Andréia B; Abraham, Wolf-Rainer; Helmholtz Center for Infection Research, Chemical Microbiology, Inhoffenstrasse 7, 38124 Braunschweig, Germany. (2010-09)
      A Gram-negative, rod-shaped, non-spore-forming bacterial strain, designated LMG 2337(T), was isolated from the blood of a patient with endocarditis and characterized. The strain was affiliated with the alphaproteobacterial genus Brevundimonas, with Brevundimonas diminuta LMG 2089(T) (98.3 % 16S rRNA gene sequence similarity) and Brevundimonas terrae KSL-145(T) (97.5 %) as its closest relatives. This affiliation was supported by chemotaxonomic data: the G+C content was 66.3 mol %, the major polar lipids were phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphatidyl glucopyranosyl diacylglycerol and the major fatty acids were summed feature 7 (one or more of C(18 : 1)ω 7c, C(18 : 1)ω 9t and C(18 : 1)ω 12t) and C(16 : 0). Strain LMG 2337(T) displayed an unusually broad substrate spectrum. The results from DNA-DNA hybridization and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain LMG 2337(T) from all of the type strains of hitherto-described Brevundimonas species. The strain therefore represents a novel species, for which the name Brevundimonas vancanneytii sp. nov. is proposed, with type strain LMG 2337(T) (=CCUG 1797(T) =ATCC 14736(T)).
    • Sphingobium aromaticiconvertens sp. nov., a xenobiotic-compound-degrading bacterium from polluted river sediment.

      Wittich, Rolf-Michael; Busse, Hans-Jürgen; Kämpfer, Peter; Tiirola, Marja; Wieser, Monika; Macedo, Alexandre J; Abraham, Wolf-Rainer; Helmholtz Zentrum für Infektionsforschung (formerly GBF), Division Microbiology, D-38124 Braunschweig, Germany. (2007-02)
      A bacterial strain capable of degrading some monochlorinated dibenzofurans, designated RW16T, was isolated from aerobic River Elbe sediments. The strain was characterized based on 16S rRNA gene sequence analysis, DNA G+C content, physiological characteristics, polyamines, ubiquinone and polar lipid pattern and fatty acid composition. This analysis revealed that strain RW16T represents a novel species of the genus Sphingobium. The DNA G+C content of strain RW16T, 60.7 mol%, is the lowest yet reported for the genus. 16S rRNA gene sequence analysis placed strain RW16T as an outlier in the genus Sphingobium. The name Sphingobium aromaticiconvertens sp. nov. is proposed for this dibenzofuran-mineralizing organism, with type strain RW16T (=DSM 12677T=CIP 109198T).
    • Sphingomonas fennica sp. nov. and Sphingomonas haloaromaticamans sp. nov., outliers of the genus Sphingomonas.

      Wittich, Rolf-Michael; Busse, Hans-Jürgen; Kämpfer, Peter; Macedo, Alexandre J; Tiirola, Marja; Wieser, Monika; Abraham, Wolf-Rainer; Bereich Mikrobiologie, Helmholtz Zentrum für Infektionsforschung (formerly GBF), D-38124 Braunschweig, Germany. (2007-08)
      Bacterial isolates obtained from polychlorophenol-contaminated sites in Finland (strain K101(T)) and from a Dutch drinking water well (strain A175(T)) were characterized taxonomically. 16S rRNA gene sequence analysis, determination of DNA G+C content, physiological characterization, estimation of the ubiquinone and polar lipid patterns and fatty acid content revealed that strains K101(T) and A175(T) were similar to Sphingomonas wittichii RW1(T) but also showed pronounced differences. The DNA G+C contents of the two novel strains were 63.6 and 66.1 mol%, respectively. On the basis of these results, two novel species of the genus Sphingomonas are described, for which the names Sphingomonas haloaromaticamans sp. nov. [type strain A175(T) (=DSM 13477(T)=CCUG 53463(T))] and Sphingomonas fennica sp. nov. [type strain K101(T) (=DSM 13665(T)=CCUG 53462(T))] are proposed.