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dc.contributor.authorTomić, Adriana
dc.contributor.authorVaranasi, Pavankumar R
dc.contributor.authorGolemac, Mijo
dc.contributor.authorMalić, Suzana
dc.contributor.authorRiese, Peggy
dc.contributor.authorBorst, Eva M
dc.contributor.authorMischak-Weissinger, Eva
dc.contributor.authorGuzmán, Carlos A
dc.contributor.authorKrmpotić, Astrid
dc.contributor.authorJonjić, Stipan
dc.contributor.authorMesserle, Martin
dc.date.accessioned2017-01-09T15:08:43Z
dc.date.available2017-01-09T15:08:43Z
dc.date.issued2016-12
dc.identifier.citationActivation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand. 2016, 12 (12):e1006015 PLoS Pathog.en
dc.identifier.issn1553-7374
dc.identifier.pmid27907183
dc.identifier.doi10.1371/journal.ppat.1006015
dc.identifier.urihttp://hdl.handle.net/10033/620686
dc.description.abstractDevelopment of an effective vaccine against human cytomegalovirus (HCMV) is a need of utmost medical importance. Generally, it is believed that a live attenuated vaccine would best provide protective immunity against this tenacious pathogen. Here, we propose a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses. An HCMV strain expressing the host ligand ULBP2 for the NKG2D receptor was found to be susceptible to control by natural killer (NK) cells, and preserved the ability to stimulate HCMV-specific T cells. Infection with the ULBP2-expressing HCMV strain caused diminished cell surface levels of MHC class I molecules. While expression of the NKG2D ligand increased the cytolytic activity of NK cells, NKG2D engagement in CD8+ T cells provided co-stimulation and compensated for lower MHC class I expression. Altogether, our data indicate that triggering of both arms of the immune system is a promising approach applicable to the generation of a live attenuated HCMV vaccine.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleActivation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.en
dc.typeArticleen
dc.contributor.departmentHel,holtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen
refterms.dateFOA2018-06-12T17:32:33Z
html.description.abstractDevelopment of an effective vaccine against human cytomegalovirus (HCMV) is a need of utmost medical importance. Generally, it is believed that a live attenuated vaccine would best provide protective immunity against this tenacious pathogen. Here, we propose a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses. An HCMV strain expressing the host ligand ULBP2 for the NKG2D receptor was found to be susceptible to control by natural killer (NK) cells, and preserved the ability to stimulate HCMV-specific T cells. Infection with the ULBP2-expressing HCMV strain caused diminished cell surface levels of MHC class I molecules. While expression of the NKG2D ligand increased the cytolytic activity of NK cells, NKG2D engagement in CD8+ T cells provided co-stimulation and compensated for lower MHC class I expression. Altogether, our data indicate that triggering of both arms of the immune system is a promising approach applicable to the generation of a live attenuated HCMV vaccine.


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