Identification of high-affinity PB1-derived peptides with enhanced affinity to the PA protein of influenza A virus polymerase.
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Authors
Wunderlich, KerstinJuozapaitis, Mindaugas
Ranadheera, Charlene
Kessler, Ulrich
Martin, Arnold
Eisel, Jessica
Beutling, Ulrike
Frank, Ronald
Schwemmle, Martin
Issue Date
2011-02
Metadata
Show full item recordAbstract
The influenza A virus polymerase complex, consisting of the subunits PB1, PB2, and PA, represents a promising target for the development of new antiviral drugs. We have previously demonstrated the feasibility of targeting the protein-protein interaction domain between PA and PB1 using peptides derived from the extreme N terminus of PB1 (amino acids [aa] 1 to 15), comprising the PA-binding domain of PB1. To increase the binding affinity of these peptides, we performed a systematic structure-affinity relationship analysis. Alanine and aspartic acid scans revealed that almost all amino acids in the core binding region (aa 5 to 11) are indispensable for PA binding. Using a library of immobilized peptides representing all possible single amino acid substitutions, we were able to identify amino acid positions outside the core PA-binding region (aa 1, 3, 12, 14, and 15) that are variable and can be replaced by affinity-enhancing residues. Surface plasmon resonance binding studies revealed that combination of several affinity-enhancing mutations led to an additive effect. Thus, the feasibility to enhance the PA-binding affinity presents an intriguing possibility to increase antiviral activity of the PB1-derived peptide and one step forward in the development of an antiviral drug against influenza A viruses.Citation
Identification of high-affinity PB1-derived peptides with enhanced affinity to the PA protein of influenza A virus polymerase. 2011, 55 (2):696-702 Antimicrob. Agents Chemother.Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.PubMed ID
21135188Type
ArticleLanguage
enISSN
1098-6596ae974a485f413a2113503eed53cd6c53
10.1128/AAC.01419-10
Scopus Count
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