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dc.contributor.authorPaulus, Constanze
dc.contributor.authorRebets, Yuriy
dc.contributor.authorTokovenko, Bogdan
dc.contributor.authorNadmid, Suvd
dc.contributor.authorTerekhova, Larisa P
dc.contributor.authorMyronovskyi, Maksym
dc.contributor.authorZotchev, Sergey B
dc.contributor.authorRückert, Christian
dc.contributor.authorBraig, Simone
dc.contributor.authorZahler, Stefan
dc.contributor.authorKalinowski, Jörn
dc.contributor.authorLuzhetskyy, Andriy N
dc.date.accessioned2017-07-05T14:47:26Z
dc.date.available2017-07-05T14:47:26Z
dc.date.issued2017-02-10
dc.identifier.citationNew natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18. 2017, 7:42382 Sci Repen
dc.identifier.issn2045-2322
dc.identifier.pmid28186197
dc.identifier.doi10.1038/srep42382
dc.identifier.urihttp://hdl.handle.net/10033/620999
dc.description.abstractMarine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleNew natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalScientific reportsen
refterms.dateFOA2018-06-12T23:31:45Z
html.description.abstractMarine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites.


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