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dc.contributor.authorLaass, Sebastian
dc.contributor.authorKleist, Sarah
dc.contributor.authorBill, Nelli
dc.contributor.authorDrüppel, Katharina
dc.contributor.authorKossmehl, Sebastian
dc.contributor.authorWöhlbrand, Lars
dc.contributor.authorRabus, Ralf
dc.contributor.authorKlein, Johannes
dc.contributor.authorRohde, Manfred
dc.contributor.authorBartsch, Annekathrin
dc.contributor.authorWittmann, Christoph
dc.contributor.authorSchmidt-Hohagen, Kerstin
dc.contributor.authorTielen, Petra
dc.contributor.authorJahn, Dieter
dc.contributor.authorSchomburg, Dietmar
dc.date.accessioned2017-07-25T14:37:36Z
dc.date.available2017-07-25T14:37:36Z
dc.date.issued2014-05-09
dc.identifier.citationGene regulatory and metabolic adaptation processes of Dinoroseobacter shibae DFL12T during oxygen depletion. 2014, 289 (19):13219-31 J. Biol. Chem.en
dc.identifier.issn1083-351X
dc.identifier.pmid24648520
dc.identifier.doi10.1074/jbc.M113.545004
dc.identifier.urihttp://hdl.handle.net/10033/621020
dc.description.abstractMetabolic flexibility is the key to the ecological success of the marine Roseobacter clade bacteria. We investigated the metabolic adaptation and the underlying changes in gene expression of Dinoroseobacter shibae DFL12(T) to anoxic life by a combination of metabolome, proteome, and transcriptome analyses. Time-resolved studies during continuous oxygen depletion were performed in a chemostat using nitrate as the terminal electron acceptor. Formation of the denitrification machinery was found enhanced on the transcriptional and proteome level, indicating that D. shibae DFL12(T) established nitrate respiration to compensate for the depletion of the electron acceptor oxygen. In parallel, arginine fermentation was induced. During the transition state, growth and ATP concentration were found to be reduced, as reflected by a decrease of A578 values and viable cell counts. In parallel, the central metabolism, including gluconeogenesis, protein biosynthesis, and purine/pyrimidine synthesis was found transiently reduced in agreement with the decreased demand for cellular building blocks. Surprisingly, an accumulation of poly-3-hydroxybutanoate was observed during prolonged incubation under anoxic conditions. One possible explanation is the storage of accumulated metabolites and the regeneration of NADP(+) from NADPH during poly-3-hydroxybutanoate synthesis (NADPH sink). Although D. shibae DFL12(T) was cultivated in the dark, biosynthesis of bacteriochlorophyll was increased, possibly to prepare for additional energy generation via aerobic anoxygenic photophosphorylation. Overall, oxygen depletion led to a metabolic crisis with partly blocked pathways and the accumulation of metabolites. In response, major energy-consuming processes were reduced until the alternative respiratory denitrification machinery was operative.
dc.language.isoenen
dc.subject.meshAdaptation, Physiologicalen
dc.subject.meshBacterial Proteinsen
dc.subject.meshDenitrificationen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshOxygen Consumptionen
dc.subject.meshRhodobacteraceaeen
dc.titleGene regulatory and metabolic adaptation processes of Dinoroseobacter shibae DFL12T during oxygen depletion.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalThe Journal of biological chemistryen
refterms.dateFOA2018-06-12T22:38:49Z
html.description.abstractMetabolic flexibility is the key to the ecological success of the marine Roseobacter clade bacteria. We investigated the metabolic adaptation and the underlying changes in gene expression of Dinoroseobacter shibae DFL12(T) to anoxic life by a combination of metabolome, proteome, and transcriptome analyses. Time-resolved studies during continuous oxygen depletion were performed in a chemostat using nitrate as the terminal electron acceptor. Formation of the denitrification machinery was found enhanced on the transcriptional and proteome level, indicating that D. shibae DFL12(T) established nitrate respiration to compensate for the depletion of the electron acceptor oxygen. In parallel, arginine fermentation was induced. During the transition state, growth and ATP concentration were found to be reduced, as reflected by a decrease of A578 values and viable cell counts. In parallel, the central metabolism, including gluconeogenesis, protein biosynthesis, and purine/pyrimidine synthesis was found transiently reduced in agreement with the decreased demand for cellular building blocks. Surprisingly, an accumulation of poly-3-hydroxybutanoate was observed during prolonged incubation under anoxic conditions. One possible explanation is the storage of accumulated metabolites and the regeneration of NADP(+) from NADPH during poly-3-hydroxybutanoate synthesis (NADPH sink). Although D. shibae DFL12(T) was cultivated in the dark, biosynthesis of bacteriochlorophyll was increased, possibly to prepare for additional energy generation via aerobic anoxygenic photophosphorylation. Overall, oxygen depletion led to a metabolic crisis with partly blocked pathways and the accumulation of metabolites. In response, major energy-consuming processes were reduced until the alternative respiratory denitrification machinery was operative.


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