Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractA large majority of genome-encrypted chemical diversity in actinobacteria remains to be discovered, which is related to the low level of secondary metabolism genes expression. Here, we report the application of a reporter-guided screening strategy to activate cryptic polycyclic tetramate macrolactam gene clusters in Streptomyces albus J1074. The analysis of the S. albus transcriptome revealed an overall low level of secondary metabolism genes transcription. Combined with transposon mutagenesis, reporter-guided screening resulted in the selection of two S. albus strains with altered secondary metabolites production. Transposon insertion in the most prominent strain, S. albus ATGSal2P2::TN14, was mapped to the XNR_3174 gene encoding an unclassified transcriptional regulator. The mutant strain was found to produce the avenolide-like compound butenolide 4. The deletion of the gene encoding a putative acyl-CoA oxidase, an orthologue of the Streptomyces avermitilis avenolide biosynthesis enzyme, in the S. albus XNR_3174 mutant caused silencing of secondary metabolism. The homologues of XNR_3174 and the butenolide biosynthesis genes were found in the genomes of multiple Streptomyces species. This result leads us to believe that the discovered regulatory elements comprise a new condition-dependent system that controls secondary metabolism in actinobacteria and can be manipulated to activate cryptic biosynthetic pathways.
CitationIdentification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074. 2017, 7 (1):9784 Sci Rep
AffiliationHelmholtz-Institut für pharmazeutische Forschung Saarland,Universitätscampus E8.1, 66123 Saarbrücken, Germany.
The following license files are associated with this item:
- Butenolides from Streptomyces albus J1074 Act as External Signals To Stimulate Avermectin Production in Streptomyces avermitilis.
- Authors: Nguyen TB, Kitani S, Shimma S, Nihira T
- Issue date: 2018 May 1
- Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.
- Authors: Bilyk B, Horbal L, Luzhetskyy A
- Issue date: 2017 Jan 4
- Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074.
- Authors: Olano C, García I, González A, Rodriguez M, Rozas D, Rubio J, Sánchez-Hidalgo M, Braña AF, Méndez C, Salas JA
- Issue date: 2014 May
- Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters.
- Authors: Kallifidas D, Jiang G, Ding Y, Luesch H
- Issue date: 2018 Feb 17
- Insights into naturally minimised Streptomyces albus J1074 genome.
- Authors: Zaburannyi N, Rabyk M, Ostash B, Fedorenko V, Luzhetskyy A
- Issue date: 2014 Feb 5