A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins.
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AbstractMany pathogenic bacteria utilize specialized secretion systems to deliver proteins called effectors into eukaryotic cells for manipulation of host pathways. The vast majority of known effector targets are host proteins, whereas a potential targeting of host nucleic acids remains little explored. There is only one family of effectors known to target DNA directly, and effectors binding host RNA are unknown. Here, we take a two-pronged approach to search for RNA-binding effectors, combining biocomputational prediction of RNA-binding domains (RBDs) in a newly assembled comprehensive dataset of bacterial secreted proteins, and experimental screening for RNA binding in mammalian cells. Only a small subset of effectors were predicted to carry an RBD, indicating that if RNA targeting was common, it would likely involve new types of RBDs. Our experimental evaluation of effectors with predicted RBDs further argues for a general paucity of RNA binding activities amongst bacterial effectors. We obtained evidence that PipB2 and Lpg2844, effector proteins of Salmonella and Legionella species, respectively, may harbor novel biochemical activities. Our study presenting the first systematic evaluation of the RNA-targeting potential of bacterial effectors offers a basis for discussion of whether or not host RNA is a prominent target of secreted bacterial proteins.
CitationA systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins. 2017, 7 (1):9328 Sci Rep
AffiliationHelmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße2, 97080 Würzburg, Germany.
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- Authors: Costa SC, Schmitz AM, Jahufar FF, Boyd JD, Cho MY, Glicksman MA, Lesser CF
- Issue date: 2012
- Identification of Novel Host Interactors of Effectors Secreted by <i>Salmonella</i> and <i>Citrobacter</i>.
- Authors: Sontag RL, Nakayasu ES, Brown RN, Niemann GS, Sydor MA, Sanchez O, Ansong C, Lu SY, Choi H, Valleau D, Weitz KK, Savchenko A, Cambronne ED, Adkins JN
- Issue date: 2016 Jul-Aug
- Exploitation of eukaryotic subcellular targeting mechanisms by bacterial effectors.
- Authors: Hicks SW, Galán JE
- Issue date: 2013 May
- New players in the same old game: a system level in silico study to predict type III secretion system and effector proteins in bacterial genomes reveals common themes in T3SS mediated pathogenesis.
- Authors: Sadarangani V, Datta S, Arunachalam M
- Issue date: 2013 Jul 26
- Quantitative mass spectrometry catalogues Salmonella pathogenicity island-2 effectors and identifies their cognate host binding partners.
- Authors: Auweter SD, Bhavsar AP, de Hoog CL, Li Y, Chan YA, van der Heijden J, Lowden MJ, Coombes BK, Rogers LD, Stoynov N, Foster LJ, Finlay BB
- Issue date: 2011 Jul 8