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dc.contributor.authorvan Ham, Marco
dc.contributor.authorTeich, René
dc.contributor.authorPhilipsen, Lars
dc.contributor.authorNiemz, Jana
dc.contributor.authorAmsberg, Nicole
dc.contributor.authorWissing, Josef
dc.contributor.authorNimtz, Manfred
dc.contributor.authorGröbe, Lothar
dc.contributor.authorKliche, Stefanie
dc.contributor.authorThiel, Nadine
dc.contributor.authorKlawonn, Frank
dc.contributor.authorHubo, Mario
dc.contributor.authorJonuleit, Helmut
dc.contributor.authorReichardt, Peter
dc.contributor.authorMüller, Andreas J
dc.contributor.authorHuehn, Jochen
dc.contributor.authorJänsch, Lothar
dc.date.accessioned2017-09-28T12:14:50Z
dc.date.available2017-09-28T12:14:50Z
dc.date.issued2017-08-17
dc.identifier.citationTCR signalling network organization at the immunological synapses of murine regulatory T cells. 2017 Eur. J. Immunol.en
dc.identifier.issn1521-4141
dc.identifier.pmid28833060
dc.identifier.doi10.1002/eji.201747041
dc.identifier.urihttp://hdl.handle.net/10033/621126
dc.description.abstractRegulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTCR signalling network organization at the immunological synapses of murine regulatory T cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zetrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalEuropean journal of immunologyen
refterms.dateFOA2018-06-13T21:23:07Z
html.description.abstractRegulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells.


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