Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function.
dc.contributor.author | van der Vlugt, L E P M | |
dc.contributor.author | Obieglo, K | |
dc.contributor.author | Ozir-Fazalalikhan, A | |
dc.contributor.author | Sparwasser, Tim | |
dc.contributor.author | Haeberlein, S | |
dc.contributor.author | Smits, H H | |
dc.date.accessioned | 2017-10-24T09:26:47Z | |
dc.date.available | 2017-10-24T09:26:47Z | |
dc.date.issued | 2017-08 | |
dc.identifier.citation | Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. 2017, 47 (9):545-554 Int. J. Parasitol. | en |
dc.identifier.issn | 1879-0135 | |
dc.identifier.pmid | 28385494 | |
dc.identifier.doi | 10.1016/j.ijpara.2017.02.002 | |
dc.identifier.uri | http://hdl.handle.net/10033/621144 | |
dc.description.abstract | Chronic schistosome infections protect against allergic airway inflammation (AAI) via the induction of IL-10-producing splenic regulatory B (Breg) cells. Previous experiments have demonstrated that schistosome-induced pulmonary B cells can also reduce AAI, but act independently of IL-10. We have now further characterized the phenotype and inhibitory activity of these protective pulmonary B cells. We excluded a role for regulatory T (Treg) cell induction as putative AAI-protective mechanisms. Schistosome-induced B cells showed increased CD86 expression and reduced cytokine expression in response to Toll-like receptor (TLR) ligands compared with control B cells. To investigate the consequences for T cell activation we cultured ovalbumin (OVA)-pulsed, schistosome-induced B cells with OVA-specific transgenic T cells and observed less Th2 cytokine expression and T cell proliferation compared with control conditions. This suppressive effect was preserved even under optimal T cell stimulation by anti-CD3/28. Blocking of the inhibitory cytokines IL-10 or TGF-β only marginally restored Th2 cytokine induction. These data suggest that schistosome-induced pulmonary B cells are impaired in their capacity to produce cytokines to TLR ligands and to induce Th2 cytokine responses independent of their antigen-presenting function. These findings underline the presence of distinct B cell subsets with different stimulatory or inhibitory properties even if induced by the same type of helminth. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. | en |
dc.type | Article | en |
dc.contributor.department | TwinCore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany. | en |
dc.identifier.journal | International journal for parasitology | en |
refterms.dateFOA | 2018-08-15T00:00:00Z | |
html.description.abstract | Chronic schistosome infections protect against allergic airway inflammation (AAI) via the induction of IL-10-producing splenic regulatory B (Breg) cells. Previous experiments have demonstrated that schistosome-induced pulmonary B cells can also reduce AAI, but act independently of IL-10. We have now further characterized the phenotype and inhibitory activity of these protective pulmonary B cells. We excluded a role for regulatory T (Treg) cell induction as putative AAI-protective mechanisms. Schistosome-induced B cells showed increased CD86 expression and reduced cytokine expression in response to Toll-like receptor (TLR) ligands compared with control B cells. To investigate the consequences for T cell activation we cultured ovalbumin (OVA)-pulsed, schistosome-induced B cells with OVA-specific transgenic T cells and observed less Th2 cytokine expression and T cell proliferation compared with control conditions. This suppressive effect was preserved even under optimal T cell stimulation by anti-CD3/28. Blocking of the inhibitory cytokines IL-10 or TGF-β only marginally restored Th2 cytokine induction. These data suggest that schistosome-induced pulmonary B cells are impaired in their capacity to produce cytokines to TLR ligands and to induce Th2 cytokine responses independent of their antigen-presenting function. These findings underline the presence of distinct B cell subsets with different stimulatory or inhibitory properties even if induced by the same type of helminth. |
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