Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine.
dc.contributor.author | Monjas, Leticia | |
dc.contributor.author | Swier, Lotteke J Y M | |
dc.contributor.author | Setyawati, Inda | |
dc.contributor.author | Slotboom, Dirk J | |
dc.contributor.author | Hirsch, Anna K H | |
dc.date.accessioned | 2017-11-08T13:08:39Z | |
dc.date.available | 2017-11-08T13:08:39Z | |
dc.date.issued | 2017-10-20 | |
dc.identifier.citation | Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine. 2017, 12 (20):1693-1696 ChemMedChem | en |
dc.identifier.issn | 1860-7187 | |
dc.identifier.pmid | 28960943 | |
dc.identifier.doi | 10.1002/cmdc.201700440 | |
dc.identifier.uri | http://hdl.handle.net/10033/621171 | |
dc.description.abstract | We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | ATP-Binding Cassette Transporters | en |
dc.subject.mesh | Bacterial Proteins | en |
dc.subject.mesh | Biological Transport | en |
dc.subject.mesh | Carrier Proteins | en |
dc.subject.mesh | Combinatorial Chemistry Techniques | en |
dc.subject.mesh | Drug Design | en |
dc.subject.mesh | Lactococcus lactis | en |
dc.subject.mesh | Models, Molecular | en |
dc.subject.mesh | Molecular Structure | en |
dc.subject.mesh | Protein Subunits | en |
dc.subject.mesh | Small Molecule Libraries | en |
dc.subject.mesh | Thiamine | en |
dc.title | Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz-Institut für pharmazeutische Forschung Saarland, Universitätscampus E9.1, 66123 Saarbrücken, Germany. | en |
dc.identifier.journal | ChemMedChem | en |
refterms.dateFOA | 2018-06-13T14:07:13Z | |
html.description.abstract | We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target. |