A metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs).
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Lassek, ChristianBurghartz, Melanie
Chaves-Moreno, Diego
Otto, Andreas
Hentschker, Christian
Fuchs, Stephan
Bernhardt, Jörg
Jauregui, Ruy
Neubauer, Rüdiger
Becher, Dörte
Pieper, Dietmar H
Jahn, Martina
Jahn, Dieter
Riedel, Katharina
Issue Date
2015-04
Metadata
Show full item recordAbstract
Long-term catheterization inevitably leads to a catheter-associated bacteriuria caused by multispecies bacterial biofilms growing on and in the catheters. The overall goal of the presented study was (1) to unravel bacterial community structure and function of such a uropathogenic biofilm and (2) to elucidate the interplay between bacterial virulence and the human immune system within the urine. To this end, a metaproteomics approach combined with in vitro proteomics analyses was employed to investigate both, the pro- and eukaryotic protein inventory. Our proteome analyses demonstrated that the biofilm of the investigated catheter is dominated by three bacterial species, that is, Pseudomonas aeruginosa, Morganella morganii, and Bacteroides sp., and identified iron limitation as one of the major challenges in the bladder environment. In vitro proteome analysis of P. aeruginosa and M. morganii isolated from the biofilm revealed that these opportunistic pathogens are able to overcome iron restriction via the production of siderophores and high expression of corresponding receptors. Notably, a comparison of in vivo and in vitro protein profiles of P. aeruginosa and M. morganii also indicated that the bacteria employ different strategies to adapt to the urinary tract. Although P. aeruginosa seems to express secreted and surface-exposed proteases to escape the human innate immune system and metabolizes amino acids, M. morganii is able to take up sugars and to degrade urea. Most interestingly, a comparison of urine protein profiles of three long-term catheterized patients and three healthy control persons demonstrated the elevated level of proteins associated with neutrophils, macrophages, and the complement system in the patient's urine, which might point to a specific activation of the innate immune system in response to biofilm-associated urinary tract infections. We thus hypothesize that the often asymptomatic nature of catheter-associated urinary tract infections might be based on a fine-tuned balance between the expression of bacterial virulence factors and the human immune system.Citation
A metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). 2015, 14 (4):989-1008 Mol. Cell ProteomicsAffiliation
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.PubMed ID
25673765Type
ArticleLanguage
enISSN
1535-9484ae974a485f413a2113503eed53cd6c53
10.1074/mcp.M114.043463
Scopus Count
Collections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
Related articles
- Azithromycin-Ciprofloxacin-Impregnated Urinary Catheters Avert Bacterial Colonization, Biofilm Formation, and Inflammation in a Murine Model of Foreign-Body-Associated Urinary Tract Infections Caused by Pseudomonas aeruginosa.
- Authors: Saini H, Vadekeetil A, Chhibber S, Harjai K
- Issue date: 2017 Mar
- Crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control.
- Authors: Broomfield RJ, Morgan SD, Khan A, Stickler DJ
- Issue date: 2009 Oct
- The Effect of Urinary Catheters on Microbial Biofilms and Catheter Associated Urinary Tract Infections.
- Authors: Kirmusaoglu S, Yurdugül S, Metin A, Vehid S
- Issue date: 2017 Mar 16
- Catheter-associated urinary tract infection by Pseudomonas aeruginosa is mediated by exopolysaccharide-independent biofilms.
- Authors: Cole SJ, Records AR, Orr MW, Linden SB, Lee VT
- Issue date: 2014 May
- Tamm-Horsfall protein facilitates catheter associated urinary tract infection.
- Authors: Raffi HS, Bates JM, Flournoy DJ, Kumar S
- Issue date: 2012 Sep 26