N-Acetylmuramic Acid (MurNAc) Auxotrophy of the Oral PathogenTannerella forsythia: Characterization of a MurNAc Kinase and Analysis of Its Role in Cell Wall Metabolism.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Mayer, Valentina M T
Tomek, Markus B
Calvert, Matthew B
MetadataShow full item record
AbstractTannerella forsythia is an anaerobic, Gram-negative oral pathogen that thrives in multispecies gingival biofilms associated with periodontitis. The bacterium is auxotrophic for the commonly essential bacterial cell wall sugarN-acetylmuramic acid (MurNAc) and, thus, strictly depends on an exogenous supply of MurNAc for growth and maintenance of cell morphology. A MurNAc transporter (Tf_MurT; Tanf_08375) and an ortholog of theEscherichia colietherase MurQ (Tf_MurQ; Tanf_08385) converting MurNAc-6-phosphate to GlcNAc-6-phosphate were recently described forT. forsythia.In between the respective genes on theT. forsythiagenome, a putative kinase gene is located. In this study, the putative kinase (Tf_MurK; Tanf_08380) was produced as a recombinant protein and biochemically characterized. Kinetic studies revealed Tf_MurK to be a 6-kinase with stringent substrate specificity for MurNAc exhibiting a 6 × 104-fold higher catalytic efficiency (kcat/Km) for MurNAc than forN-acetylglucosamine (GlcNAc) withkcatvalues of 10.5 s-1and 0.1 s-1andKmvalues of 200 μM and 116 mM, respectively. The enzyme kinetic data suggest that Tf_MurK is subject to substrate inhibition (Ki[S]= 4.2 mM). To assess the role of Tf_MurK in the cell wall metabolism ofT. forsythia, a kinase deletion mutant (ΔTf_murK::erm) was constructed. This mutant accumulated MurNAc intracellularly in the exponential phase, indicating the capability to take up MurNAc, but inability to catabolize MurNAc. In the stationary phase, the MurNAc level was reduced in the mutant, while the level of the peptidoglycan precursor UDP-MurNAc-pentapeptide was highly elevated. Further, according to scanning electron microscopy evidence, theΔTf_murK::ermmutant was more tolerant toward low MurNAc concentration in the medium (below 0.5 μg/ml) before transition from healthy, rod-shaped to fusiform cells occurred, while the parent strain required > 1 μg/ml MurNAc for optimal growth. These data reveal thatT. forsythiareadily catabolizes exogenous MurNAc but simultaneously channels a proportion of the sugar into peptidoglycan biosynthesis. Deletion ofTf_murKblocks MurNAc catabolism and allows the direction of MurNAc solely to peptidoglycan biosynthesis, resulting in a growth advantage in MurNAc-depleted medium. This work increases our understanding of theT. forsythiacell wall metabolism and may pave new routes for lead finding in the treatment of periodontitis.
CitationN-Acetylmuramic Acid (MurNAc) Auxotrophy of the Oral PathogenTannerella forsythia: Characterization of a MurNAc Kinase and Analysis of Its Role in Cell Wall Metabolism. 2018, 9:19 Front Microbiol
AffiliationHIPS, Helmholtz-Institut für pharmazeutische Forschung Saarland, Universitätscampus 8.1, 66123 Saarbrücken, Germany.
JournalFrontiers in microbiology
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Identification of a Novel N-Acetylmuramic Acid Transporter in Tannerella forsythia.
- Authors: Ruscitto A, Hottmann I, Stafford GP, Schäffer C, Mayer C, Sharma A
- Issue date: 2016 Nov 15
- Peptidoglycan Recycling in Gram-Positive Bacteria Is Crucial for Survival in Stationary Phase.
- Authors: Borisova M, Gaupp R, Duckworth A, Schneider A, Dalügge D, Mühleck M, Deubel D, Unsleber S, Yu W, Muth G, Bischoff M, Götz F, Mayer C
- Issue date: 2016 Oct 11
- Peptidoglycan synthesis in Tannerella forsythia: Scavenging is the modus operandi.
- Authors: Ruscitto A, Sharma A
- Issue date: 2018 Apr
- The <i>N</i>-Acetylmuramic Acid 6-Phosphate Phosphatase MupP Completes the <i>Pseudomonas</i> Peptidoglycan Recycling Pathway Leading to Intrinsic Fosfomycin Resistance.
- Authors: Borisova M, Gisin J, Mayer C
- Issue date: 2017 Mar 28
- Recovery of the Peptidoglycan Turnover Product Released by the Autolysin Atl in <i>Staphylococcus aureus</i> Involves the Phosphotransferase System Transporter MurP and the Novel 6-phospho-<i>N</i>-acetylmuramidase MupG.
- Authors: Kluj RM, Ebner P, Adamek M, Ziemert N, Mayer C, Borisova M
- Issue date: 2018