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dc.contributor.authorVillacorta, Luisen
dc.contributor.authorMinarrieta, Luciaen
dc.contributor.authorSalvatore, Sonia Ren
dc.contributor.authorKhoo, Nicholas Ken
dc.contributor.authorRom, Orenen
dc.contributor.authorGao, Zhenen
dc.contributor.authorBerman, Rebecca Cen
dc.contributor.authorJobbagy, Somaen
dc.contributor.authorLi, Lihuaen
dc.contributor.authorWoodcock, Steven Ren
dc.contributor.authorChen, Y Eugeneen
dc.contributor.authorFreeman, Bruce Aen
dc.contributor.authorFerreira, Ana Men
dc.contributor.authorSchopfer, Francisco Jen
dc.contributor.authorVitturi, Dario Aen
dc.date.accessioned2018-03-19T15:56:35Z
dc.date.available2018-03-19T15:56:35Z
dc.date.issued2018-05
dc.identifier.citationIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. 2018, 15:522-531 Redox Biolen
dc.identifier.issn2213-2317
dc.identifier.pmid29413964
dc.identifier.doi10.1016/j.redox.2018.01.005
dc.identifier.urihttp://hdl.handle.net/10033/621325
dc.description.abstractConjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitro-conjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.en
dc.typeArticleen
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalRedox biologyen
refterms.dateFOA2018-06-13T05:28:21Z
html.description.abstractConjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitro-conjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.


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