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dc.contributor.authorStichling, Nicoleen
dc.contributor.authorSuomalainen, Maariten
dc.contributor.authorFlatt, Justin Wen
dc.contributor.authorSchmid, Markusen
dc.contributor.authorPacesa, Martinen
dc.contributor.authorHemmi, Silvioen
dc.contributor.authorJungraithmayr, Wolfgangen
dc.contributor.authorMaler, Mareike Den
dc.contributor.authorFreudenberg, Marina Aen
dc.contributor.authorPlückthun, Andreasen
dc.contributor.authorMay, Tobiasen
dc.contributor.authorKöster, Marioen
dc.contributor.authorFejer, Györgyen
dc.contributor.authorGreber, Urs Fen
dc.date.accessioned2018-03-23T15:32:45Z
dc.date.available2018-03-23T15:32:45Z
dc.date.issued2018-03
dc.identifier.citationLung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor. 2018, 14 (3):e1006914 PLoS Pathog.en
dc.identifier.issn1553-7374
dc.identifier.pmid29522575
dc.identifier.doi10.1371/journal.ppat.1006914
dc.identifier.urihttp://hdl.handle.net/10033/621333
dc.description.abstractMacrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleLung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen
refterms.dateFOA2018-06-13T14:13:27Z
html.description.abstractMacrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses.


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