Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractBacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers. Precise genome engineering using bacteriophage recombinases is particularly useful for genome mining. However, recombinases are usually host-specific. The genome-guided discovery of novel recombinases and their transient expression could boost cryptic BGC mining. Herein, we reported a genetic system employing Red recombinases from Burkholderiales strain DSM 7029 for efficient genome engineering in several Burkholderiales species that currently lack effective genetic tools. Using specialized recombinases-assisted in situ insertion of functional promoters, we successfully mined five cryptic nonribosomal peptide synthetase/polyketide synthase BGCs, two of which were silent. Two classes of lipopeptides, glidopeptins and rhizomides, were identified through extensive spectroscopic characterization. This recombinase expression strategy offers utility within other bacteria species, allowing bioprospecting for potentially scalable discovery of novel metabolites with attractive bioactivities.
AffiliationHIPS, Helmholtz-Institute für pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
- Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole-Genome Bacterial Artificial Chromosome Library in Streptomyces spp.
- Authors: Xu M, Wang Y, Zhao Z, Gao G, Huang SX, Kang Q, He X, Lin S, Pang X, Deng Z, Tao M
- Issue date: 2016 Oct 1
- Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster in <i>Streptomyces</i> Species.
- Authors: Nah HJ, Pyeon HR, Kang SH, Choi SS, Kim ES
- Issue date: 2017
- Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining.
- Authors: Tang X, Li J, Millán-Aguiñaga N, Zhang JJ, O'Neill EC, Ugalde JA, Jensen PR, Mantovani SM, Moore BS
- Issue date: 2015 Dec 18
- New strategies and approaches for engineering biosynthetic gene clusters of microbial natural products.
- Authors: Li L, Jiang W, Lu Y
- Issue date: 2017 Dec
- Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029.
- Authors: Bian X, Tang B, Yu Y, Tu Q, Gross F, Wang H, Li A, Fu J, Shen Y, Li YZ, Stewart AF, Zhao G, Ding X, Müller R, Zhang Y
- Issue date: 2017 Jul 21