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dc.contributor.authorWang, Xue
dc.contributor.authorZhou, Haibo
dc.contributor.authorChen, Hanna
dc.contributor.authorJing, Xiaoshu
dc.contributor.authorZheng, Wentao
dc.contributor.authorLi, Ruijuan
dc.contributor.authorSun, Tao
dc.contributor.authorLiu, Jiaqi
dc.contributor.authorFu, Jun
dc.contributor.authorHuo, Liujie
dc.contributor.authorLi, Yue-Zhong
dc.contributor.authorShen, Yuemao
dc.contributor.authorDing, Xiaoming
dc.contributor.authorMüller, Rolf
dc.contributor.authorBian, Xiaoying
dc.contributor.authorZhang, Youming
dc.date.accessioned2018-06-11T13:03:58Z
dc.date.available2018-06-11T13:03:58Z
dc.date.issued2018-05-01
dc.identifier.pmid29666226
dc.identifier.urihttp://hdl.handle.net/10033/621394
dc.description.abstractBacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers. Precise genome engineering using bacteriophage recombinases is particularly useful for genome mining. However, recombinases are usually host-specific. The genome-guided discovery of novel recombinases and their transient expression could boost cryptic BGC mining. Herein, we reported a genetic system employing Red recombinases from Burkholderiales strain DSM 7029 for efficient genome engineering in several Burkholderiales species that currently lack effective genetic tools. Using specialized recombinases-assisted in situ insertion of functional promoters, we successfully mined five cryptic nonribosomal peptide synthetase/polyketide synthase BGCs, two of which were silent. Two classes of lipopeptides, glidopeptins and rhizomides, were identified through extensive spectroscopic characterization. This recombinase expression strategy offers utility within other bacteria species, allowing bioprospecting for potentially scalable discovery of novel metabolites with attractive bioactivities.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectBurkholderialesen_US
dc.subjectgenome miningen_US
dc.subjectlipopeptideen_US
dc.subjectnatural producten_US
dc.subjectrecombinasesen_US
dc.titleDiscovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institute für pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en_US
refterms.dateFOA2018-06-11T13:03:58Z


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