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dc.contributor.authorNeumann-Schaal, Meina
dc.contributor.authorMetzendorf, Nicole G
dc.contributor.authorTroitzsch, Daniel
dc.contributor.authorNuss, Aaron Mischa
dc.contributor.authorHofmann, Julia Danielle
dc.contributor.authorBeckstette, Michael
dc.contributor.authorDersch, Petra
dc.contributor.authorOtto, Andreas
dc.contributor.authorSievers, Susanne
dc.date.accessioned2018-06-26T13:40:17Z
dc.date.available2018-06-26T13:40:17Z
dc.date.issued2018-05-31
dc.identifier.issn1095-8274
dc.identifier.pmid29859941
dc.identifier.doi10.1016/j.anaerobe.2018.05.018
dc.identifier.urihttp://hdl.handle.net/10033/621411
dc.description.abstractClostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells with only few changes in the metabolite pattern. However, an extensive change in gene expression was determined by RNA-Seq. The most striking adaptation strategies involve a change in the reductive fermentation pathways of the amino acids proline, glycine and leucine. But also a far-reaching restructuring in the carbohydrate metabolism was detected with changes in the phosphotransferase system (PTS) facilitated uptake of sugars and a repression of enzymes of glycolysis and butyrate fermentation. Furthermore, a temporary induction in the synthesis of cofactor riboflavin was detected possibly due to an increased demand for flavin mononucleotid (FMN) and flavin adenine dinucleotide (FAD) in redox reactions. However, biosynthesis of the cofactors thiamin pyrophosphate and cobalamin were repressed deducing oxidation-prone enzymes and intermediates in these pathways. Micro-aerobically shocked cells were characterized by an increased demand for cysteine and a thiol redox proteomics approach revealed a dramatic increase in the oxidative state of cysteine in more than 800 peptides after 15 min of micro-aerobic shock. This provides not only a catalogue of oxidation-prone cysteine residues in the C. difficile proteome but also puts the amino acid cysteine into a key position in the oxidative stress response. Our study suggests that tolerance of C. difficile towards Oen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectClostridioides difficileen_US
dc.subjectCysteine modificationen_US
dc.subjectMetabolomicsen_US
dc.subjectOxidative stressen_US
dc.subjectRNA-Seqen_US
dc.subjectRedox proteomicsen_US
dc.titleTracking gene expression and oxidative damage of O-stressed Clostridioides difficile by a multi-omics approach.en_US
dc.typeArticleen_US
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en_US
dc.source.journaltitleAnaerobe


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