Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors.
dc.contributor.author | Eicke, Dorothee | |
dc.contributor.author | Baigger, Anja | |
dc.contributor.author | Schulze, Kai | |
dc.contributor.author | Latham, Sharissa L | |
dc.contributor.author | Halloin, Caroline | |
dc.contributor.author | Zweigerdt, Robert | |
dc.contributor.author | Guzman, Carlos A | |
dc.contributor.author | Blasczyk, Rainer | |
dc.contributor.author | Figueiredo, Constança | |
dc.date.accessioned | 2018-08-07T09:25:04Z | |
dc.date.available | 2018-08-07T09:25:04Z | |
dc.date.issued | 2018-07-05 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.pmid | 29977045 | |
dc.identifier.doi | 10.1038/s41598-018-28459-x | |
dc.identifier.uri | http://hdl.handle.net/10033/621437 | |
dc.description.abstract | Megakaryocytes (MKs) are the precursors of platelets (PLTs) and may be used for PLT production in vivo or in vitro, as well as a source for PLT-derived growth factors. Induced pluripotent stem cells represent an unlimited cell source for the in vitro production of MKs. This study aimed at developing an effective, xeno-free and scalable system to produce high numbers of MKs. In particular, microcarrier beads-assisted stirred bioreactors were evaluated as a means of improving MK yields. This method resulted in the production of 18.7 × 10 | en_US |
dc.language.iso | en | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
dc.title | Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors. | en_US |
dc.type | Article | en_US |
dc.contributor.department | Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany. | en_US |
refterms.dateFOA | 2018-08-07T09:25:04Z | |
dc.source.journaltitle | Scientific reports |