Show simple item record

dc.contributor.authorOlsowski, Maike
dc.contributor.authorHoffmann, Frederike
dc.contributor.authorHain, Andrea
dc.contributor.authorKirchhoff, Lisa
dc.contributor.authorTheegarten, Dirk
dc.contributor.authorTodt, Daniel
dc.contributor.authorSteinmann, Eike
dc.contributor.authorBuer, Jan
dc.contributor.authorRath, Peter-Michael
dc.contributor.authorSteinmann, Joerg
dc.date.accessioned2018-11-13T12:37:38Z
dc.date.available2018-11-13T12:37:38Z
dc.date.issued2018-08-24
dc.identifier.issn2045-2322
dc.identifier.pmid30143674
dc.identifier.doi10.1038/s41598-018-30909-5
dc.identifier.urihttp://hdl.handle.net/10033/621556
dc.description.abstractExophiala dermatitidis causes chromoblastomycosis, phaeohyphomycosis and fatal infections of the central nervous system of patients with Asian background. It is also found in respiratory secretions from cystic fibrosis (CF) patients. In this study a variety of E. dermatitidis strains (isolates from Asia, environmental and CF) were characterized in their pathogenicity by survival analyzes using two different invertebrate host organisms, Caenorhabditis elegans and Galleria mellonella. Furthermore, the morphological development of hyphal formation was analyzed. E. dermatitidis exhibited pathogenicity in C. elegans. The virulence varied in a strain-dependent manner, but the nematodes were a limited model to study hyphal formation. Analysis of a melanin-deficient mutant (Mel-3) indicates that melanin plays a role during virulence processes in C. elegans. The strains isolated from Asian patients exhibited significantly higher virulence in G. mellonella compared to strains from other sources. Histological analyzes also revealed a higher potential of invasive hyphal growth in strains isolated from Asian patients. Interestingly, no significant difference was found in virulence between the Mel-3 mutant and their wild type counterpart during infection in G. mellonella. In conclusion, invasive hyphal formation of E. dermatitidis was associated with increased virulence. This work is the basis for future studies concerning E. dermatitidis virulence.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleExophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence.en_US
dc.typeArticleen_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
refterms.dateFOA2018-11-13T12:37:38Z
dc.source.journaltitleScientific reports


Files in this item

Thumbnail
Name:
Olsowski et al.pdf
Size:
2.309Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States