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dc.contributor.authorTawk, Caroline
dc.contributor.authorNigro, Giulia
dc.contributor.authorRodrigues Lopes, Ines
dc.contributor.authorAguilar, Carmen
dc.contributor.authorLisowski, Clivia
dc.contributor.authorMano, Miguel
dc.contributor.authorSansonetti, Philippe
dc.contributor.authorVogel, Jörg
dc.contributor.authorEulalio, Ana
dc.date.accessioned2018-12-03T10:43:31Z
dc.date.available2018-12-03T10:43:31Z
dc.date.issued2018-11-02
dc.identifier.issn1460-2075
dc.identifier.pmid30389666
dc.identifier.doi10.15252/embj.201798529
dc.identifier.urihttp://hdl.handle.net/10033/621595
dc.description.abstractWhile mucosal inflammation is a major source of stress during enteropathogen infection, it remains to be fully elucidated how the host benefits from this environment to clear the pathogen. Here, we show that host stress induced by different stimuli mimicking inflammatory conditions strongly reduces the binding of Shigella flexneri to epithelial cells. Mechanistically, stress activates acid sphingomyelinase leading to host membrane remodeling. Consequently, knockdown or pharmacological inhibition of the acid sphingomyelinase blunts the stress-dependent inhibition of Shigella binding to host cells. Interestingly, stress caused by intracellular Shigella replication also results in remodeling of the host cell membrane, in vitro and in vivo, which precludes re-infection by this and other non-motile pathogens. In contrast, Salmonella Typhimurium overcomes the shortage of permissive entry sites by gathering effectively at the remaining platforms through its flagellar motility. Overall, our findings reveal host membrane remodeling as a novel stress-responsive cell-autonomous defense mechanism that protects epithelial cells from infection by non-motile bacterial pathogens.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectSalmonellaen_US
dc.subjectShigellaen_US
dc.subjectacid sphingomyelinaseen_US
dc.subjecthost stress responseen_US
dc.subjectmembrane remodelingen_US
dc.titleStress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
refterms.dateFOA2018-05-02T00:00:00Z
dc.source.journaltitleThe EMBO journal


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