Show simple item record

dc.contributor.authorVillegas-Plazas, Marcela
dc.contributor.authorWos-Oxley, Melissa L
dc.contributor.authorSanchez, Juan A
dc.contributor.authorPieper, Dietmar H
dc.contributor.authorThomas, Olivier P
dc.contributor.authorJunca, Howard
dc.date.accessioned2018-12-03T13:34:04Z
dc.date.available2018-12-03T13:34:04Z
dc.date.issued2018-11-10
dc.identifier.issn1432-184X
dc.identifier.pmid30413836
dc.identifier.doi10.1007/s00248-018-1285-y
dc.identifier.urihttp://hdl.handle.net/10033/621596
dc.description.abstractXestospongia muta is among the most emblematic sponge species inhabiting coral reefs of the Caribbean Sea. Besides being the largest sponge species growing in the Caribbean, it is also known to produce secondary metabolites. This study aimed to assess the effect of depth and season on the symbiotic bacterial dynamics and major metabolite profiles of specimens of X. muta thriving in a tropical marine biome (Portobelo Bay, Panamá), which allow us to determine whether variability patterns are similar to those reported for subtropical latitudes. The bacterial assemblages were characterized using Illumina deep-sequencing and metabolomic profiles using UHPLC-DAD-ELSD from five depths (ranging 9-28 m) across two seasons (spring and autumn). Diverse symbiotic communities, representing 24 phyla with a predominance of Proteobacteria and Chloroflexi, were found. Although several thousands of OTUs were determined, most of them belong to the rare biosphere and only 23 to a core community. There was a significant difference between the structure of the microbial communities in respect to season (autumn to spring), with a further significant difference between depths only in autumn. This was partially mirrored in the metabolome profile, where the overall metabolite composition did not differ between seasons, but a significant depth gradient was observed in autumn. At the phyla level, Cyanobacteria, Firmicutes, Actinobacteria, and Spirochaete showed a mild-moderate correlation with the metabolome profile. The metabolomic profiles were mainly characterized by known brominated polyunsaturated fatty acids. This work presents findings about the composition and dynamics of the microbial assemblages of X. muta expanding and confirming current knowledge about its remarkable diversity and geographic variability as observed in this tropical marine biome.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject16S rRNA geneen_US
dc.subjectDepth gradienten_US
dc.subjectHolobionten_US
dc.subjectIllumina deep-sequencingen_US
dc.subjectMarine spongesen_US
dc.subjectMetabolomicsen_US
dc.subjectSeasonen_US
dc.subjectSymbiotic bacterial communitiesen_US
dc.subjectXestospongia mutaen_US
dc.titleVariations in Microbial Diversity and Metabolite Profiles of the Tropical Marine Sponge Xestospongia muta with Season and Depth.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.source.journaltitleMicrobial ecology


Files in this item

Thumbnail
Name:
Villegas-Plazas et al.pdf
Size:
1.188Mb
Format:
PDF
Description:
original manuscript with figures

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International