On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractLamellipodia are flat membrane protrusions formed during mesenchymal motion. Polymerization at the leading edge assembles the actin filament network and generates protrusion force. How this force is supported by the network and how the assembly rate is shared between protrusion and network retrograde flow determines the protrusion rate. We use mathematical modeling to understand experiments changing the F-actin density in lamellipodia of B16-F1 melanoma cells by modulation of Arp2/3 complex activity or knockout of the formins FMNL2 and FMNL3. Cells respond to a reduction of density with a decrease of protrusion velocity, an increase in the ratio of force to filament number, but constant network assembly rate. The relation between protrusion force and tension gradient in the F-actin network and the density dependency of friction, elasticity, and viscosity of the network explain the experimental observations. The formins act as filament nucleators and elongators with differential rates. Modulation of their activity suggests an effect on network assembly rate. Contrary to these expectations, the effect of changes in elongator composition is much weaker than the consequences of the density change. We conclude that the force acting on the leading edge membrane is the force required to drive F-actin network retrograde flow.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
PublisherAmrican Society for Cell biology
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- FMNL formins boost lamellipodial force generation.
- Authors: Kage F, Winterhoff M, Dimchev V, Mueller J, Thalheim T, Freise A, Brühmann S, Kollasser J, Block J, Dimchev G, Geyer M, Schnittler HJ, Brakebusch C, Stradal TE, Carlier MF, Sixt M, Käs J, Faix J, Rottner K
- Issue date: 2017 Mar 22
- Efficiency of lamellipodia protrusion is determined by the extent of cytosolic actin assembly.
- Authors: Dimchev G, Steffen A, Kage F, Dimchev V, Pernier J, Carlier MF, Rottner K
- Issue date: 2017 May 15
- FMNL2 drives actin-based protrusion and migration downstream of Cdc42.
- Authors: Block J, Breitsprecher D, Kühn S, Winterhoff M, Kage F, Geffers R, Duwe P, Rohn JL, Baum B, Brakebusch C, Geyer M, Stradal TE, Faix J, Rottner K
- Issue date: 2012 Jun 5
- Protrusion and actin assembly are coupled to the organization of lamellar contractile structures.
- Authors: Lim JI, Sabouri-Ghomi M, Machacek M, Waterman CM, Danuser G
- Issue date: 2010 Aug 1
- Modeling of protrusion phenotypes driven by the actin-membrane interaction.
- Authors: Enculescu M, Sabouri-Ghomi M, Danuser G, Falcke M
- Issue date: 2010 Apr 21