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dc.contributor.authorYuyama, Kamila Tomoko
dc.contributor.authorWendt, Lucile
dc.contributor.authorSurup, Frank
dc.contributor.authorKretz, Robin
dc.contributor.authorChepkirui, Clara
dc.contributor.authorWittstein, Kathrin
dc.contributor.authorBoonlarppradab, Chollaratt
dc.contributor.authorWongkanoun, Sarunyou
dc.contributor.authorLuangsa-Ard, Jennifer
dc.contributor.authorStadler, Marc
dc.contributor.authorAbraham, Wolf-Rainer
dc.date.accessioned2019-01-10T09:51:58Z
dc.date.available2019-01-10T09:51:58Z
dc.date.issued2018-10-30
dc.identifier.citationBiomolecules. 2018 Oct 30;8(4). pii: biom8040129. doi: 10.3390/biom8040129en_US
dc.identifier.issn2218-273X
dc.identifier.pmid30380779
dc.identifier.doi10.3390/biom8040129
dc.identifier.urihttp://hdl.handle.net/10033/621640
dc.description.abstractDuring the course of our ongoing work to discover new inhibitors of biofilm formation of Staphylococcus aureus from fungal sources, we observed biofilm inhibition by cytochalasans isolated from cultures of the ascomycete Hypoxylon fragiforme for the first time. Two new compounds were purified by a bioassay-guided fractionation procedure; their structures were elucidated subsequently by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). This unexpected finding prompted us to test further cytochalasans from other fungi and from commercial sources for comparison. Out of 21 cytochalasans, 13 showed significant inhibition of Staphylococcus aureus biofilm formation at subtoxic levels. These findings indicate the potential of cytochalasans as biofilm inhibitors for the first time, also because the minimum inhibitory concentrations (MIC) are independent of the anti-biofilm activities. However, cytochalasans are known to be inhibitors of actin, making some of them very toxic for eukaryotic cells. Since the chemical structures of the tested compounds were rather diverse, the inclusion of additional derivatives, as well as the evaluation of their selectivity against mammalian cells vs. the bacterium, will be necessary as next step in order to develop structure-activity relationships and identify the optimal candidates for development of an anti-biofilm agent. View Full-Texten_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectXylarialesen_US
dc.subjectascomycotaen_US
dc.subjectbacterial pathogensen_US
dc.subjectbiofilm dispersionen_US
dc.subjectchromatographyen_US
dc.subjectnatural productsen_US
dc.subjectsecondary metabolitesen_US
dc.subjectstructure elucidationen_US
dc.titleCytochalasans Act as Inhibitors of Biofilm Formation of Staphylococcus Aureus.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalBiomoleculesen_US
refterms.dateFOA2019-01-10T09:51:59Z
dc.source.journaltitleBiomolecules


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