Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3
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Issue Date
2018-09-25
Metadata
Show full item recordAbstract
Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.Publisher
SpringerJournal
Indian Journal of MicrobiologyAdditional Links
http://link.springer.com/10.1007/s12088-018-0761-xType
ArticleISSN
0046-89910973-7715
0-7084-0623-8
ae974a485f413a2113503eed53cd6c53
10.1007/s12088-018-0761-x
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