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dc.contributor.authorKuzhyk, Yuriy
dc.contributor.authorLopatniuk, Maria
dc.contributor.authorLuzhetskyy, Andriy
dc.contributor.authorFedorenko, Victor
dc.contributor.authorOstash, Bohdan
dc.date.accessioned2019-01-10T13:21:57Z
dc.date.available2019-01-10T13:21:57Z
dc.date.issued2018-09-25
dc.identifier.issn0046-8991
dc.identifier.issn0973-7715
dc.identifier.issn0-7084-0623-8
dc.identifier.doi10.1007/s12088-018-0761-x
dc.identifier.urihttp://hdl.handle.net/10033/621641
dc.description.abstractHere we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.en_US
dc.publisherSpringeren_US
dc.relation.urlhttp://link.springer.com/10.1007/s12088-018-0761-xen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectStreptomyces ghanaensisen_US
dc.subjectMoenomycinsen_US
dc.subjectGenome engineeringen_US
dc.subjectntegrative vectorsen_US
dc.titleGenome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalIndian Journal of Microbiologyen_US
dc.source.volume59
dc.source.issue1
dc.source.beginpage109
dc.source.endpage111
dc.source.journaltitleIndian Journal of Microbiology


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International