Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.
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Issue Date
2019-01-01
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Show full item recordAbstract
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.Citation
Methods Mol Biol. 2019;1923:309-322. doi: 10.1007/978-1-4939-9024-5_14.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
Humana PressJournal
Methods in molecular biologyPubMed ID
30737747Type
ArticleLanguage
enISSN
1940-6029ae974a485f413a2113503eed53cd6c53
10.1007/978-1-4939-9024-5_14
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