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dc.contributor.authorChen, Linhai
dc.contributor.authorKeller, Laura J
dc.contributor.authorCordasco, Edward A
dc.contributor.authorBogyo, Matthew
dc.contributor.authorLentz, Christian S
dc.date.accessioned2019-03-14T08:51:02Z
dc.date.available2019-03-14T08:51:02Z
dc.date.issued2019-02-15
dc.identifier.citationAngew Chem Int Ed Engl. 2019 Feb 15. doi: 10.1002/anie.201900511.en_US
dc.identifier.issn1521-3773
dc.identifier.pmid30768830
dc.identifier.doi10.1002/anie.201900511
dc.identifier.urihttp://hdl.handle.net/10033/621721
dc.description.abstractPhenotypically distinct cellular (sub)populations are clinically relevant for virulence and antibiotic resistance of a bacterial pathogen, but functionally different cells are usually indistinguishable from each other. Here, we introduce fluorescent activity-based probes as chemical tools for single-cell phenotypic characterization of enzyme activity levels in Staphylococcus aureus. We screened a 1,2,3-triazole urea library to identify selective inhibitors of fluorophosphonate-binding serine hydrolases and lipases in S. aureus and synthesized target-selective activity-based probes. Molecular imaging and activity-based protein profiling studies with these probes revealed a dynamic network within this enzyme family involving compensatory regulation of specific family members and exposed single-cell phenotypic heterogeneity. We propose chemical probe labeling of enzymatic activities as a generalizable method for phenotyping of bacterial cells at the population and single-cell level.en_US
dc.publisherWiley-Blackwellen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectActivity-based protein profilingen_US
dc.subjectMolecular Imagingen_US
dc.subjectPhenotypeen_US
dc.subjectenzymesen_US
dc.subjectsingle-cell analysisen_US
dc.titleSingle-cell phenotypic characterization of Staphylococcus aureus with fluorescent triazole urea activity-based probes.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig Germany.en_US
dc.identifier.journalAngewandte Chemie - International Editionen_US
dc.identifier.pmcidPMC6456404en
refterms.dateFOA2020-02-15T00:00:00Z
dc.source.journaltitleAngewandte Chemie (International ed. in English)


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International