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dc.contributor.authorSchaks, Matthias
dc.contributor.authorDöring, Hermann
dc.contributor.authorKage, Frieda
dc.contributor.authorSteffen, Anika
dc.contributor.authorKlünemann, Thomas
dc.contributor.authorBlankenfeldt, Wulf
dc.contributor.authorStradal, Theresia
dc.contributor.authorRottner, Klemens
dc.date.accessioned2019-09-05T13:20:47Z
dc.date.available2019-09-05T13:20:47Z
dc.date.issued2019-08-26
dc.identifier.citationSmall GTPases. 2019 Aug 26:1-11. doi: 10.1080/21541248.2019.1657755.en_US
dc.identifier.issn2154-1256
dc.identifier.pmid31451035
dc.identifier.doi10.1080/21541248.2019.1657755
dc.identifier.urihttp://hdl.handle.net/10033/621927
dc.description.abstractCell migration frequently involves the formation of lamellipodial protrusions, the initiation of which requires Rac GTPases signalling to heteropentameric WAVE regulatory complex (WRC). While Rac-related RhoG and Cdc42 can potently stimulate lamellipodium formation, so far presumed to occur by upstream signalling to Rac activation, we show here that the latter can be bypassed by RhoG and Cdc42 given that WRC has been artificially activated. This evidence arises from generation of B16-F1 cells simultaneously lacking both Rac GTPases and WRC, followed by reconstitution of lamellipodia formation with specific Rho-GTPase and differentially active WRC variant combinations. We conclude that formation of canonical lamellipodia requires WRC activation through Rac, but can possibly be tuned, in addition, by WRC interactions with RhoG and Cdc42.en_US
dc.language.isoenen_US
dc.publisherTaylor and Francisen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectArp2/3 complexen_US
dc.subjectCRISPR/Cas9en_US
dc.subjectRho-GTPaseen_US
dc.subjectblebbingen_US
dc.subjectfilopodiumen_US
dc.subjectlamellipodiumen_US
dc.subjectmigrationen_US
dc.subjectprotrusionen_US
dc.subjectstress fibreen_US
dc.titleRhoG and Cdc42 can contribute to Rac-dependent lamellipodia formation through WAVE regulatory complex-binding.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalSmall GTPasesen_US
refterms.dateFOA2019-09-05T13:20:48Z
dc.source.journaltitleSmall GTPases


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