Impact of healthy aging on active bacterial assemblages throughout the gastrointestinal tract.
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Authors
Schütte, KerstinSchulz, Christian
Vilchez-Vargas, Ramiro
Vasapolli, Riccardo
Palm, Frederike
Simon, Bianca
Schomburg, Dirk
Lux, Anke
Geffers, Robert
Pieper, Dietmar H
Link, Alexander
Malfertheiner, Peter
Issue Date
2021-08-30
Metadata
Show full item recordAbstract
The adaption of gut microbiota (GM) throughout human life is a key factor in maintaining health. Interventions to restore a healthy GM composition may have the potential to improve health and disease outcomes in the elderly. We performed a comprehensive characterization of changes in the luminal and mucosa-associated microbiota composition in elderly compared with younger healthy individuals. Samples from saliva and feces, and biopsies from the upper and lower gastrointestinal tract (UGIT, LGIT), were collected from 59 asymptomatic individuals grouped by age: 40-55, 56-70, and 71-85 years). All underwent anthropometric, geriatric, and nutritional assessment. RNA was extracted and reverse-transcribed into complementary DNA; the V1-V2 regions of 16S ribosomal RNA genes were amplified and sequenced. Abundances of the taxa in all taxonomic ranks in each sample type were used to construct sample-similarity matrices by the Bray-Curtis algorithm. Significant differences between defined groups were assessed by analysis of similarity. The bacterial community showed strong interindividual variations and a clear distinction between samples from UGIT, LGIT, and feces. While in saliva some taxa were affected by aging, this number was considerably greater in UGIT and was subsequently higher in LGIT. Unexpectedly, aging scarcely influenced the bacterial community of feces over the age range of 40-85 years. The development of interventions to preserve and restore human health with increased age by establishing a healthy gut microbiome should not rely solely on data from fecal analysis, as the intestinal mucosa is affected by more significant changes, which differ from those observed in fecal analyses.Citation
Gut Microbes. 2021 Jan-Dec;13(1):1966261. doi: 10.1080/19490976.2021.1966261.Affiliation
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.Publisher
Taylor & FrancisJournal
Gut microbesPubMed ID
34455919Type
ArticleLanguage
enEISSN
1949-0984ae974a485f413a2113503eed53cd6c53
10.1080/19490976.2021.1966261
Scopus Count
The following license files are associated with this item:
- Creative Commons
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