Identification of cardiac malformations in mice lacking Ptdsr using a novel high-throughput magnetic resonance imaging technique
Name:
schneider et al_final.pdf
Size:
3.400Mb
Format:
PDF
Description:
Open Access publication
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Schneider, Jürgen EBöse, Jens
Bamforth, Simon D
Gruber, Achim D
Broadbent, Carol
Clarke, Kieran
Neubauer, Stefan
Lengeling, Andreas
Bhattacharya, Shoumo
Issue Date
2004-12-22
Metadata
Show full item recordAbstract
Background Congenital heart defects are the leading non-infectious cause of death in children. Genetic studies in the mouse have been crucial to uncover new genes and signaling pathways associated with heart development and congenital heart disease. The identification of murine models of congenital cardiac malformations in high-throughput mutagenesis screens and in gene-targeted models is hindered by the opacity of the mouse embryo. Results We developed and optimized a novel method for high-throughput multi-embryo magnetic resonance imaging (MRI). Using this approach we identified cardiac malformations in phosphatidylserine receptor (Ptdsr) deficient embryos. These included ventricular septal defects, double-outlet right ventricle, and hypoplasia of the pulmonary artery and thymus. These results indicate that Ptdsr plays a key role in cardiac development. Conclusions Our novel multi-embryo MRI technique enables high-throughput identification of murine models for human congenital cardiopulmonary malformations at high spatial resolution. The technique can be easily adapted for mouse mutagenesis screens and, thus provides an important new tool for identifying new mouse models for human congenital heart diseases.Citation
BMC Developmental Biology 2004 4:16Publisher
BioMed CentralPubMed ID
15615595PubMed Central ID
545075Additional Links
http://www.biomedcentral.com/1471-213X/4/16http://creativecommons.org/licenses/by/2.0
Language
en_USISSN
1471-213Xae974a485f413a2113503eed53cd6c53
10.1186/1471-213X-4-16
Scopus Count
The following license files are associated with this item:
Related articles
- Rapid identification and 3D reconstruction of complex cardiac malformations in transgenic mouse embryos using fast gradient echo sequence magnetic resonance imaging.
- Authors: Schneider JE, Bamforth SD, Farthing CR, Clarke K, Neubauer S, Bhattacharya S
- Issue date: 2003 Feb
- High-throughput analysis of mouse embryos by magnetic resonance imaging.
- Authors: Bamforth SD, Schneider JE, Bhattacharya S
- Issue date: 2012 Jan 1
- Images in cardiovascular medicine. Assessment of double chamber right ventricle by magnetic resonance imaging.
- Authors: Ibrahim T, Dennig K, Schwaiger M, Schömig A
- Issue date: 2002 Jun 4
- Case 119: Double-chambered right ventricle.
- Authors: Kardux JJ, van Es HW, Rensing BJ
- Issue date: 2007 Aug
- Morphological evaluation of atrioventricular septal defects by magnetic resonance imaging.
- Authors: Parsons JM, Baker EJ, Anderson RH, Ladusans EJ, Hayes A, Qureshi SA, Deverall PB, Fagg N, Cook A, Maisey MN
- Issue date: 1990 Aug