Intrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Heroven, Ann Kathrin
MetadataShow full item record
AbstractPathogens, which alternate between environmental reservoirs and a mammalian host, frequently use thermal sensing devices to adjust virulence gene expression. Here, we identify the Yersinia virulence regulator RovA as a protein thermometer. Thermal shifts encountered upon host entry lead to a reversible conformational change of the autoactivator, which reduces its DNA-binding functions and renders it more susceptible for proteolysis. Cooperative binding of RovA to its target promoters is significantly reduced at 37 degrees C, indicating that temperature control of rovA transcription is primarily based on the autoregulatory loop. Thermally induced reduction of DNA-binding is accompanied by an enhanced degradation of RovA, primarily by the Lon protease. This process is also subject to growth phase control. Studies with modified/chimeric RovA proteins indicate that amino acid residues in the vicinity of the central DNA-binding domain are important for proteolytic susceptibility. Our results establish RovA as an intrinsic temperature-sensing protein in which thermally induced conformational changes interfere with DNA-binding capacity, and secondarily render RovA susceptible to proteolytic degradation.
CitationIntrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA. 2009, 5 (5):e1000435 PLoS Pathog.
AffiliationInstitut für Mikrobiologie, Technische Universität Braunschweig, Germany.
The following license files are associated with this item:
- RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis.
- Authors: Heroven AK, Dersch P
- Issue date: 2006 Dec
- Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation.
- Authors: Tran HJ, Heroven AK, Winkler L, Spreter T, Beatrix B, Dersch P
- Issue date: 2005 Dec 23
- Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
- Authors: Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P
- Issue date: 2012 Oct 19
- A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence.
- Authors: Revell PA, Miller VL
- Issue date: 2000 Feb
- Environmental control of invasin expression in Yersinia pseudotuberculosis is mediated by regulation of RovA, a transcriptional activator of the SlyA/Hor family.
- Authors: Nagel G, Lahrz A, Dersch P
- Issue date: 2001 Sep