Molecular dissection of Salmonella-induced membrane ruffling versus invasion.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Stradal, Theresia E B
MetadataShow full item record
AbstractType III secretion system-mediated injection of a cocktail of bacterial proteins drives actin rearrangements, frequently adopting the shape of prominent protuberances of ruffling membrane, and culminating in host cell invasion of Gram-negative pathogens like Salmonella typhimurium. Different Salmonella effectors are able to bind actin and activate Rho-family GTPases, which have previously been implicated in mediating actin-dependent Salmonella entry by interacting with N-WASP or WAVE-complex, well-established activators of the actin nucleation machine Arp2/3-complex. Using genetic deletion and RNA interference studies, we show here that neither individual nor collective removal of these Arp2/3- complex activators affected host cell invasion as efficiently as Arp2/3-complex knock-down, although the latter was also not essential. However, interference with WAVE-complex function abrogated Salmonella-induced membrane ruffling without significantly affecting entry efficiency, actin or Arp2/3-complex accumulation. In addition, scanning electron microscopy images captured entry events in the absence of prominent membrane ruffles. Finally, localization and RNA interference studies indicated a relevant function in Salmonella entry for the novel Arp2/3-complex regulator WASH. These data establish for the first time that Salmonella invasion is separable from bacteria-induced membrane ruffling, and uncover an additional Arp2/3-complex activator as well as an Arp2/3-complex-independent actin assembly activity that contribute to Salmonella invasion.
CitationMolecular dissection of Salmonella-induced membrane ruffling versus invasion. 2010, 12 (1):84-98 Cell. Microbiol.
AffiliationHelmholtz Centre for Infection Research, Braunschweig, Germany.
CollectionsPublications of RG Cytoskeleton Dynamics (CYD)
The following license files are associated with this item:
- Analysis of the mechanisms of Salmonella-induced actin assembly during invasion of host cells and intracellular replication.
- Authors: Unsworth KE, Way M, McNiven M, Machesky L, Holden DW
- Issue date: 2004 Nov
- Activation of a RhoA/myosin II-dependent but Arp2/3 complex-independent pathway facilitates Salmonella invasion.
- Authors: Hänisch J, Kölm R, Wozniczka M, Bumann D, Rottner K, Stradal TE
- Issue date: 2011 Apr 21
- Different WASP family proteins stimulate different Arp2/3 complex-dependent actin-nucleating activities.
- Authors: Zalevsky J, Lempert L, Kranitz H, Mullins RD
- Issue date: 2001 Dec 11
- N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits.
- Authors: Benesch S, Polo S, Lai FP, Anderson KI, Stradal TE, Wehland J, Rottner K
- Issue date: 2005 Jul 15
- Regulation of Salmonella-induced membrane ruffling by SipA differs in strains lacking other effectors.
- Authors: Perrett CA, Jepson MA
- Issue date: 2009 Mar