This is the institutional Repository of the Helmholtz Centre for Infection Research in Braunschweig/Germany (HZI), the Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Saarbrücken/Germany, the TWINCORE Zentrum für Exprerimentelle und Klinische Infektionsforschung, Hannover/Germany,Helmholtz-Institut für RNA-basierte Infektionsforschung (HIRI), BRICS, CSSB and the Study Centre Hannover, Hannover/Germany.

 

  • Chronic rhinosinusitis with nasal polyps is characterized by dysbacteriosis of the nasal microbiota.

    Chalermwatanachai, Thanit; Vilchez-Vargas, Ramiro; Holtappels, Gabriele; Lacoere, Tim; Jáuregui, Ruy; Kerckhof, Frederiek-Maarten; Pieper, Dietmar H; Van de Wiele, Tom; Vaneechoutte, Mario; Van Zele, Thibaut; Bachert, Claus; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. (2018-05-21)
    Chronic rhinosinusitis with nasal polyp (CRSwNP) patients are often characterized by asthma comorbidity and a type-2 inflammation of the sinonasal mucosa. The mucosal microbiota has been suggested to be implicated in the persistence of inflammation, but associations have not been well defined. To compare the bacterial communities of healthy subjects with CRSwNP patients, we collected nasal swabs from 17 healthy subjects, 21 CRSwNP patients without asthma (CRSwNP-A), and 20 CRSwNP patients with co-morbid asthma (CRSwNP+A). We analysed the microbiota using high-throughput sequencing of the bacterial 16S rRNA. Bacterial communities were different between the three groups. Haemophilus influenzae was significantly enriched in CRSwNP patients, Propionibacterium acnes in the healthy group; Staphylococcus aureus was abundant in the CRSwNP-A group, even though present in 57% of patients. Escherichia coli was found in high amounts in CRSwNP+A patients. Nasal tissues of CRSwNP+A patients expressed significantly higher concentrations of IgE, SE-IgE, and IL-5 compared to those of CRSwNP-A patients. Co-cultivation demonstrated that P. acnes growth was inhibited by H. influenzae, E. coli and S. aureus. The nasal microbiota of healthy subjects are different from those of CRSwNP-A and CRSwNP+A patients. However, the most abundant species in healthy status could not inhibit those in CRSwNP disease.
  • Antiviral 4-Hydroxypleurogrisein and Antimicrobial Pleurotin Derivatives from Cultures of the Nematophagous Basidiomycete .

    Sandargo, Birthe; Thongbai, Benjarong; Praditya, Dimas; Steinmann, Eike; Stadler, Marc; Surup, Frank; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany; TWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany. (2018-10-19)
    4-Hydroxypleurogrisein, a congener of the anticancer-lead compound pleurotin, as well as six further derivatives were isolated from the basidiomycete Hohenbuehelia grisea, strain MFLUCC 12-0451. The structures were elucidated utilizing high resolution electron spray ionization mass spectrometry (HRESIMS) and 1D and 2D nuclear magnetic resonance (NMR) spectral data and evaluated for their biological activities; for leucopleurotin, we provide Xray data. While most congeners showed moderate antimicrobial and cytotoxic activity, 4-hydroxypleurogrisein emerged as an inhibitor of hepatitis C virus infectivity in mammalian liver cells.
  • Susceptibility of Chikungunya Virus to Inactivation by Heat and Commercially and World Health Organization-Recommended Biocides.

    Franz, Sergej; Friesland, Martina; Passos, Vânia; Todt, Daniel; Simmons, Graham; Goffinet, Christine; Steinmann, Eike; TWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany. (2018-09-22)
    Despite increasing clinical relevance of Chikungunya virus (CHIKV) infection, caused by a rapidly emerging pathogen, recommended guidelines for its inactivation do not exist. In this study, we investigated the susceptibility of CHIKV to inactivation by heat and commercially available hand, surface, and World Health Organization-recommended disinfectants to define CHIKV prevention protocols for healthcare systems.
  • Interactive Feedback of Data Quality in Clinical Research. A Case Study from an Infectious Diseases Cohort.

    Glöckner, Stephan; Schindler, Daniela; Karch, André; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. (2018-01-01)
    To improve data quality, clinical cohort studies require ongoing centralized data monitoring. Results of data quality monitoring are often reported in a general format, without individual feedback for different types of users within the research consortium. Several R packages provide the possibility for an easy to use approach to analyze and communicate information in interactive web-based forms. This article describes a pilot, which is applied in a German infectious disease cohort using interactive feedback and gamification to report data quality, to ultimately increase the quality of research outcomes.
  • The Small-Compound Inhibitor K22 Displays Broad Antiviral Activity against Different Members of the Family Flaviviridae and Offers Potential as a Panviral Inhibitor.

    García-Nicolás, Obdulio; V'kovski, Philip; Vielle, Nathalie J; Ebert, Nadine; Züst, Roland; Portmann, Jasmine; Stalder, Hanspeter; Gaschen, Véronique; Vieyres, Gabrielle; Stoffel, Michael; Schweizer, Matthias; Summerfield, Artur; Engler, Olivier; Pietschmann, Thomas; Todt, Daniel; Alves, Marco P; Thiel, Volker; Pfaender, Stephanie; TWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany. (2018-11-01)
    The virus family

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