The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate.

2.50
Hdl Handle:
http://hdl.handle.net/10033/139962
Title:
The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate.
Authors:
Lu, Xin; Sun, Jibin; Nimtz, Manfred; Wissing, Josef; Zeng, An-Ping ( 0000-0001-9768-7096 ) ; Rinas, Ursula
Abstract:
The filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thus the homologous glucoamylase (glaA) promoter as well as the glaA signal sequence are widely used for heterologous protein production. Xylose is known to strongly repress glaA expression while maltose is a potent inducer of glaA promoter controlled genes. For a more profound understanding of A. niger physiology, a comprehensive analysis of the intra- and extracellular proteome of Aspergillus niger AB1.13 growing on defined medium with xylose or maltose as carbon substrate was carried out using 2-D gel electrophoresis/Maldi-ToF and nano-HPLC MS/MS.
Affiliation:
Helmholtz Center for Infection Research, Inhoffenstr, Braunschweig, Germany.
Citation:
The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. 2010, 9:23 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2010
URI:
http://hdl.handle.net/10033/139962
DOI:
10.1186/1475-2859-9-23
PubMed ID:
20406453
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
publications of the research group compound profiling and screening (COPS)

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Xinen
dc.contributor.authorSun, Jibinen
dc.contributor.authorNimtz, Manfreden
dc.contributor.authorWissing, Josefen
dc.contributor.authorZeng, An-Pingen
dc.contributor.authorRinas, Ursulaen
dc.date.accessioned2011-08-17T13:57:00Z-
dc.date.available2011-08-17T13:57:00Z-
dc.date.issued2010-
dc.identifier.citationThe intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. 2010, 9:23 Microb. Cell Fact.en
dc.identifier.issn1475-2859-
dc.identifier.pmid20406453-
dc.identifier.doi10.1186/1475-2859-9-23-
dc.identifier.urihttp://hdl.handle.net/10033/139962-
dc.description.abstractThe filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thus the homologous glucoamylase (glaA) promoter as well as the glaA signal sequence are widely used for heterologous protein production. Xylose is known to strongly repress glaA expression while maltose is a potent inducer of glaA promoter controlled genes. For a more profound understanding of A. niger physiology, a comprehensive analysis of the intra- and extracellular proteome of Aspergillus niger AB1.13 growing on defined medium with xylose or maltose as carbon substrate was carried out using 2-D gel electrophoresis/Maldi-ToF and nano-HPLC MS/MS.en
dc.language.isoenen
dc.subject.meshAiren
dc.subject.meshAspergillus nigeren
dc.subject.meshBioreactorsen
dc.subject.meshCarbonen
dc.subject.meshFungal Proteinsen
dc.subject.meshHydrogen-Ion Concentrationen
dc.subject.meshMaltoseen
dc.subject.meshProteomeen
dc.subject.meshProteomicsen
dc.subject.meshXyloseen
dc.titleThe intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Center for Infection Research, Inhoffenstr, Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen
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