Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes.

2.50
Hdl Handle:
http://hdl.handle.net/10033/14554
Title:
Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes.
Authors:
Vonberg, Ralf-Peter; Häussler, Susanne; Vandamme, Peter; Steinmetz, Ivo
Abstract:
Members of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach was developed for the rapid identification of B. cepacia genomovar I, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (lineage III-A and III-B), Burkholderia stabilis (genomovar IV) and Burkholderia vietnamiensis (genomovar V), which cause the large majority of infections in CF patients. The method was based on the detection of differences in the recA gene sequence by using rapid-cycle PCR and genomovar-specific fluorescence resonance energy transfer (FRET) probes. The genomovar status of all 39 B. cepacia complex strains tested (genomovars I-V) was identified by melting-curve analysis. Each FRET probe produced a specific fluorescence signal only with the respective genomovar, and not with other B. cepacia complex strains and Burkholderia spp. The identification system was easy to handle and revealed B. cepacia complex genomovar I-V status from culture isolates within about 1 h.
Citation:
J. Med. Microbiol. 2006, 55(Pt 6):721-7
Issue Date:
1-Jun-2006
URI:
http://hdl.handle.net/10033/14554
DOI:
10.1099/jmm.0.46457-0
PubMed ID:
16687590
Type:
Article
Language:
en
ISSN:
0022-2615
Appears in Collections:
publications of the research group chronic pseudomonas infections (CPI)

Full metadata record

DC FieldValue Language
dc.contributor.authorVonberg, Ralf-Peter-
dc.contributor.authorHäussler, Susanne-
dc.contributor.authorVandamme, Peter-
dc.contributor.authorSteinmetz, Ivo-
dc.date.accessioned2007-11-13T14:09:50Z-
dc.date.available2007-11-13T14:09:50Z-
dc.date.issued2006-06-01-
dc.identifier.citationJ. Med. Microbiol. 2006, 55(Pt 6):721-7en
dc.identifier.issn0022-2615-
dc.identifier.pmid16687590-
dc.identifier.doi10.1099/jmm.0.46457-0-
dc.identifier.urihttp://hdl.handle.net/10033/14554-
dc.description.abstractMembers of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach was developed for the rapid identification of B. cepacia genomovar I, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (lineage III-A and III-B), Burkholderia stabilis (genomovar IV) and Burkholderia vietnamiensis (genomovar V), which cause the large majority of infections in CF patients. The method was based on the detection of differences in the recA gene sequence by using rapid-cycle PCR and genomovar-specific fluorescence resonance energy transfer (FRET) probes. The genomovar status of all 39 B. cepacia complex strains tested (genomovars I-V) was identified by melting-curve analysis. Each FRET probe produced a specific fluorescence signal only with the respective genomovar, and not with other B. cepacia complex strains and Burkholderia spp. The identification system was easy to handle and revealed B. cepacia complex genomovar I-V status from culture isolates within about 1 h.en
dc.format.extent732544 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleIdentification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes.en
dc.typeArticleen
dc.format.digYES-

Related articles on PubMed

All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.