ISPa20 advances the individual evolution of Pseudomonas aeruginosa clone C subclone C13 strains isolated from cystic fibrosis patients by insertional mutagenesis and genomic rearrangements.

2.50
Hdl Handle:
http://hdl.handle.net/10033/15013
Title:
ISPa20 advances the individual evolution of Pseudomonas aeruginosa clone C subclone C13 strains isolated from cystic fibrosis patients by insertional mutagenesis and genomic rearrangements.
Authors:
Kresse, Andreas U; Blöcker, Helmut; Römling, Ute
Abstract:
Pseudomonas aeruginosa clone C strains, which chronically colonize the lungs of cystic fibrosis patients reorganize their genome structure. In this study, a novel member of the IS3 subfamily of IS elements, ISPa20, was detected which was specific for clone C subclone C13 strains. ISPa20, which was present in high copy number, mediated events of genomic reorganization. ISPa20 was inserted into P. aeruginosa backbone genes leading to adaptation to the cystic fibrosis lung habitat and into DNA acquired through horizontal gene transfer. Further on, large chromosomal inversions were mediated by ISPa20. In contrast to strains of other subclonal linages high rates of genomic rearrangements of subclone C13 strains were observed in vitro. The acquisition of mobile elements by P. aeruginosa clone C strains in the lungs of cystic fibrosis patients supports the chronic colonization by insertional mutagenesis and chromosome restructuring leading to microevolution within clone C that reflects macroevolution observed on the species level.
Affiliation:
Research Group Clonal Variability, Division of Cell- and Immune Biology, GBF - German Research Centre for Biotechnology, Mascheroder Weg 1, 38124, Braunschweig, Germany.
Citation:
ISPa20 advances the individual evolution of Pseudomonas aeruginosa clone C subclone C13 strains isolated from cystic fibrosis patients by insertional mutagenesis and genomic rearrangements. 2006, 185 (4):245-54 Arch. Microbiol.
Journal:
Archives of microbiology
Issue Date:
May-2006
URI:
http://hdl.handle.net/10033/15013
DOI:
10.1007/s00203-006-0089-5
PubMed ID:
16474952
Type:
Article
Language:
en
ISSN:
0302-8933
Appears in Collections:
Publications of Dept. Cell Biology (ZB)

Full metadata record

DC FieldValue Language
dc.contributor.authorKresse, Andreas U-
dc.contributor.authorBlöcker, Helmut-
dc.contributor.authorRömling, Ute-
dc.date.accessioned2007-12-06T10:15:26Z-
dc.date.available2007-12-06T10:15:26Z-
dc.date.issued2006-05-
dc.identifier.citationISPa20 advances the individual evolution of Pseudomonas aeruginosa clone C subclone C13 strains isolated from cystic fibrosis patients by insertional mutagenesis and genomic rearrangements. 2006, 185 (4):245-54 Arch. Microbiol.en
dc.identifier.issn0302-8933-
dc.identifier.pmid16474952-
dc.identifier.doi10.1007/s00203-006-0089-5-
dc.identifier.urihttp://hdl.handle.net/10033/15013-
dc.description.abstractPseudomonas aeruginosa clone C strains, which chronically colonize the lungs of cystic fibrosis patients reorganize their genome structure. In this study, a novel member of the IS3 subfamily of IS elements, ISPa20, was detected which was specific for clone C subclone C13 strains. ISPa20, which was present in high copy number, mediated events of genomic reorganization. ISPa20 was inserted into P. aeruginosa backbone genes leading to adaptation to the cystic fibrosis lung habitat and into DNA acquired through horizontal gene transfer. Further on, large chromosomal inversions were mediated by ISPa20. In contrast to strains of other subclonal linages high rates of genomic rearrangements of subclone C13 strains were observed in vitro. The acquisition of mobile elements by P. aeruginosa clone C strains in the lungs of cystic fibrosis patients supports the chronic colonization by insertional mutagenesis and chromosome restructuring leading to microevolution within clone C that reflects macroevolution observed on the species level.en
dc.language.isoenen
dc.titleISPa20 advances the individual evolution of Pseudomonas aeruginosa clone C subclone C13 strains isolated from cystic fibrosis patients by insertional mutagenesis and genomic rearrangements.en
dc.typeArticleen
dc.contributor.departmentResearch Group Clonal Variability, Division of Cell- and Immune Biology, GBF - German Research Centre for Biotechnology, Mascheroder Weg 1, 38124, Braunschweig, Germany.-
dc.identifier.journalArchives of microbiologyen

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