Spatial variation of active microbiota in the rice rhizosphere revealed by in situ stable isotope probing of phospholipid fatty acids.

2.50
Hdl Handle:
http://hdl.handle.net/10033/19814
Title:
Spatial variation of active microbiota in the rice rhizosphere revealed by in situ stable isotope probing of phospholipid fatty acids.
Authors:
Lu, Yahai; Abraham, Wolf-Rainer ( 0000-0002-2850-2649 ) ; Conrad, Ralf
Abstract:
This report is part of a serial study applying stable isotope labelling to rice microcosms to track the utilization of recently photosynthesized carbon by active microbiota in the rhizosphere. The objective of the present study was to apply phospholipid fatty acid-based stable isotope probing (PLFA-SIP) to detect the spatial variation of active microorganisms associated with rhizosphere carbon flow. In total, 49 pulses of 13CO2 were applied to rice plants in a microcosm over a period of 7 days. Rhizosphere soil was separated from bulk soil by a root bag. Soil samples were taken from rhizosphere and bulk soil, and the bulk soil samples were further partitioned both vertically (up layer and down layer) and horizontally with increasing distance to the root bag. Incorporation of 13C into PLFAs sharply decreased with distance to the roots. The labelling of 16:1omega9, 18:1omega7, 18:1omega9, 18:2omega6,9 and i14:0 PLFAs was relatively stronger in the rhizosphere while that of i15:0 and i17:0 increased in the bulk soil. The microorganisms associated with 16:1omega9 were active in both up- and down-layer soils. The microorganisms represented by i14:0, 18:1omega7 and 18:2omega6,9 exhibited a relatively higher activity in up-layer soil, whereas those represented by i15:0 and i17:0 were more active in down-layer soil. These results suggest that in the rhizosphere Gram-negative and eukaryotic microorganisms were most actively assimilating root-derived C, whereas Gram-positive microorganisms became relatively more important in the bulk soil. The active populations apparently differed between up- and down-layer soil and in particular changed with distance to the roots, demonstrating systematic changes in the activity of the soil microbiota surrounding roots.
Affiliation:
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
Citation:
Spatial variation of active microbiota in the rice rhizosphere revealed by in situ stable isotope probing of phospholipid fatty acids. 2007, 9 (2):474-81 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Feb-2007
URI:
http://hdl.handle.net/10033/19814
DOI:
10.1111/j.1462-2920.2006.01164.x
PubMed ID:
17222145
Type:
Article
Language:
en
ISSN:
1462-2912
Appears in Collections:
publications of the research group chemical microbiology (CMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Yahai-
dc.contributor.authorAbraham, Wolf-Rainer-
dc.contributor.authorConrad, Ralf-
dc.date.accessioned2008-03-05T12:50:08Z-
dc.date.available2008-03-05T12:50:08Z-
dc.date.issued2007-02-
dc.identifier.citationSpatial variation of active microbiota in the rice rhizosphere revealed by in situ stable isotope probing of phospholipid fatty acids. 2007, 9 (2):474-81 Environ. Microbiol.en
dc.identifier.issn1462-2912-
dc.identifier.pmid17222145-
dc.identifier.doi10.1111/j.1462-2920.2006.01164.x-
dc.identifier.urihttp://hdl.handle.net/10033/19814-
dc.description.abstractThis report is part of a serial study applying stable isotope labelling to rice microcosms to track the utilization of recently photosynthesized carbon by active microbiota in the rhizosphere. The objective of the present study was to apply phospholipid fatty acid-based stable isotope probing (PLFA-SIP) to detect the spatial variation of active microorganisms associated with rhizosphere carbon flow. In total, 49 pulses of 13CO2 were applied to rice plants in a microcosm over a period of 7 days. Rhizosphere soil was separated from bulk soil by a root bag. Soil samples were taken from rhizosphere and bulk soil, and the bulk soil samples were further partitioned both vertically (up layer and down layer) and horizontally with increasing distance to the root bag. Incorporation of 13C into PLFAs sharply decreased with distance to the roots. The labelling of 16:1omega9, 18:1omega7, 18:1omega9, 18:2omega6,9 and i14:0 PLFAs was relatively stronger in the rhizosphere while that of i15:0 and i17:0 increased in the bulk soil. The microorganisms associated with 16:1omega9 were active in both up- and down-layer soils. The microorganisms represented by i14:0, 18:1omega7 and 18:2omega6,9 exhibited a relatively higher activity in up-layer soil, whereas those represented by i15:0 and i17:0 were more active in down-layer soil. These results suggest that in the rhizosphere Gram-negative and eukaryotic microorganisms were most actively assimilating root-derived C, whereas Gram-positive microorganisms became relatively more important in the bulk soil. The active populations apparently differed between up- and down-layer soil and in particular changed with distance to the roots, demonstrating systematic changes in the activity of the soil microbiota surrounding roots.en
dc.language.isoenen
dc.subject.meshBiodiversityen
dc.subject.meshCarbon Isotopesen
dc.subject.meshFatty Acidsen
dc.subject.meshOryza sativaen
dc.subject.meshPhospholipidsen
dc.subject.meshPlant Rootsen
dc.subject.meshSoil Microbiologyen
dc.titleSpatial variation of active microbiota in the rice rhizosphere revealed by in situ stable isotope probing of phospholipid fatty acids.en
dc.typeArticleen
dc.contributor.departmentCollege of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.en
dc.identifier.journalEnvironmental microbiologyen
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