2.50
Hdl Handle:
http://hdl.handle.net/10033/210029
Title:
High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells.
Authors:
Hemmen, Katherina; Reinl, Tobias; Buttler, Kerstin; Behler, Friederike; Dieken, Hauke; Jänsch, Lothar ( 0000-0002-5655-1181 ) ; Wilting, Jörg; Weich, Herbert A
Abstract:
Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogenesis.
Affiliation:
Department of Gene Regulation, HZI, Build. D, Inhoffenstr. 7, 38124, Braunschweig, Germany. katharina.hemmen@ewetel.net
Citation:
High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells. 2011, 14 (2):163-72 Angiogenesis
Journal:
Angiogenesis
Issue Date:
May-2011
URI:
http://hdl.handle.net/10033/210029
DOI:
10.1007/s10456-011-9200-x
PubMed ID:
21234671
Type:
Article
Language:
en
ISSN:
1573-7209
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorHemmen, Katherinaen
dc.contributor.authorReinl, Tobiasen
dc.contributor.authorButtler, Kerstinen
dc.contributor.authorBehler, Friederikeen
dc.contributor.authorDieken, Haukeen
dc.contributor.authorJänsch, Lotharen
dc.contributor.authorWilting, Jörgen
dc.contributor.authorWeich, Herbert Aen
dc.date.accessioned2012-02-08T15:14:21Zen
dc.date.available2012-02-08T15:14:21Zen
dc.date.issued2011-05en
dc.identifier.citationHigh-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells. 2011, 14 (2):163-72 Angiogenesisen
dc.identifier.issn1573-7209en
dc.identifier.pmid21234671en
dc.identifier.doi10.1007/s10456-011-9200-xen
dc.identifier.urihttp://hdl.handle.net/10033/210029en
dc.description.abstractRecently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogenesis.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshCulture Media, Conditioneden
dc.subject.meshEndothelial Cellsen
dc.subject.meshFlow Cytometryen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshLungen
dc.subject.meshMass Spectrometryen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshNeovascularization, Physiologicen
dc.subject.meshProteomeen
dc.subject.meshReproducibility of Resultsen
dc.subject.meshSolubilityen
dc.subject.meshStem Cellsen
dc.subject.meshSubcellular Fractionsen
dc.subject.meshVascular Endothelial Growth Factor Receptor-1en
dc.subject.meshVascular Endothelial Growth Factor Receptor-2en
dc.titleHigh-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Gene Regulation, HZI, Build. D, Inhoffenstr. 7, 38124, Braunschweig, Germany. katharina.hemmen@ewetel.neten
dc.identifier.journalAngiogenesisen
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