2.50
Hdl Handle:
http://hdl.handle.net/10033/24652
Title:
Rapid identification of viridans streptococci by mass spectrometric discrimination.
Authors:
Friedrichs, C; Rodloff, A C; Chhatwal, G S; Schellenberger, W; Eschrich, K
Abstract:
Viridans streptococci (VS) are responsible for several systemic diseases, such as endocarditis, abscesses, and septicemia. Unfortunately, species identification by conventional methods seems to be more difficult than species identification of other groups of bacteria. The aim of the present study was to evaluate the use of cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) for the rapid identification of 10 different species of VS. A total of 99 VS clinical isolates, 10 reference strains, and 20 strains from our in-house culture collection were analyzed by MALDI-TOF-MS. To evaluate the mass-spectrometric discrimination results, all strains were identified in parallel by phenotypic and genotypic methods. MALDI-TOF-MS identified 71 isolates as the mitis group, 23 as the anginosus group, and 5 as Streptococcus salivarius. Comparison of the species identification results obtained by the MALDI-TOF-MS analyses and with the phenotypic/genotypic identification systems showed 100% consistency at the species level. Thus, MALDI-TOF-MS seems to be a rapid and reliable method for the identification of species of VS from clinical samples.
Affiliation:
Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Liebigstr. 24, D-04105 Leipzig, Germany. claudia.friedrichs@medizin.uni-leipzig.de
Citation:
Rapid identification of viridans streptococci by mass spectrometric discrimination. 2007, 45 (8):2392-7 J. Clin. Microbiol.
Journal:
Journal of clinical microbiology
Issue Date:
Aug-2007
URI:
http://hdl.handle.net/10033/24652
DOI:
10.1128/JCM.00556-07
PubMed ID:
17553974
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17553974
Type:
Book
Language:
en
ISSN:
0095-1137
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorFriedrichs, Cen
dc.contributor.authorRodloff, A Cen
dc.contributor.authorChhatwal, G Sen
dc.contributor.authorSchellenberger, Wen
dc.contributor.authorEschrich, Ken
dc.date.accessioned2008-05-02T08:26:18Zen
dc.date.available2008-05-02T08:26:18Zen
dc.date.issued2007-08en
dc.identifier.citationRapid identification of viridans streptococci by mass spectrometric discrimination. 2007, 45 (8):2392-7 J. Clin. Microbiol.en
dc.identifier.issn0095-1137en
dc.identifier.pmid17553974en
dc.identifier.doi10.1128/JCM.00556-07en
dc.identifier.urihttp://hdl.handle.net/10033/24652en
dc.description.abstractViridans streptococci (VS) are responsible for several systemic diseases, such as endocarditis, abscesses, and septicemia. Unfortunately, species identification by conventional methods seems to be more difficult than species identification of other groups of bacteria. The aim of the present study was to evaluate the use of cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) for the rapid identification of 10 different species of VS. A total of 99 VS clinical isolates, 10 reference strains, and 20 strains from our in-house culture collection were analyzed by MALDI-TOF-MS. To evaluate the mass-spectrometric discrimination results, all strains were identified in parallel by phenotypic and genotypic methods. MALDI-TOF-MS identified 71 isolates as the mitis group, 23 as the anginosus group, and 5 as Streptococcus salivarius. Comparison of the species identification results obtained by the MALDI-TOF-MS analyses and with the phenotypic/genotypic identification systems showed 100% consistency at the species level. Thus, MALDI-TOF-MS seems to be a rapid and reliable method for the identification of species of VS from clinical samples.en
dc.language.isoenen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17553974en
dc.subject.meshBacterial Typing Techniquesen
dc.subject.meshHumansen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen
dc.subject.meshStreptococcal Infectionsen
dc.subject.meshViridans Streptococcien
dc.titleRapid identification of viridans streptococci by mass spectrometric discrimination.en
dc.typeBooken
dc.contributor.departmentInstitute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Liebigstr. 24, D-04105 Leipzig, Germany. claudia.friedrichs@medizin.uni-leipzig.deen
dc.identifier.journalJournal of clinical microbiologyen
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