High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/25714
Title:
High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells.
Authors:
Klöpper, Jonas; Lindenmaier, Werner; Fiedler, Ulrike; Mehlhorn, Alexander; Stark, G Björn; Finkenzeller, Günter
Abstract:
Survival of ex vivo constructed tissues after transplantation is limited by insufficient oxygen and nutrient supply. Therefore, strategies aiming at improvement of neovascularization of engineered tissues are a key issue in tissue engineering applications. This in vitro study aimed at exploring the usability of osteogenically differentiated human mesenchymal stem cells (MSCs) as carriers of the angiogenic growth factor genes vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) for therapeutic angiogenesis in bone tissue engineering. The ex vivo adenoviral vector mediated transduction into osteogenically differentiated MSCs revealed a highly efficient and long lasting expression of the transgenes. Biological activity of VEGF and Ang-1 secreted from transduced cells was confirmed by analyzing the sprouting, proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) in response to conditioned medium obtained from transduced cells. The transduced osteogenically differentiated MSCs described in this report may be suitable for inducing neovascularization in bone tissue engineering applications.
Affiliation:
Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, Germany.
Citation:
High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells. 2008, 75 (1):83-90 Microvasc. Res.
Journal:
Microvascular research
Issue Date:
Jan-2008
URI:
http://hdl.handle.net/10033/25714
DOI:
10.1016/j.mvr.2007.04.010
PubMed ID:
17603084
Type:
Article
Language:
en
ISSN:
0026-2862
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorKlöpper, Jonas-
dc.contributor.authorLindenmaier, Werner-
dc.contributor.authorFiedler, Ulrike-
dc.contributor.authorMehlhorn, Alexander-
dc.contributor.authorStark, G Björn-
dc.contributor.authorFinkenzeller, Günter-
dc.date.accessioned2008-05-13T10:42:15Z-
dc.date.available2008-05-13T10:42:15Z-
dc.date.issued2008-01-
dc.identifier.citationHigh efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells. 2008, 75 (1):83-90 Microvasc. Res.en
dc.identifier.issn0026-2862-
dc.identifier.pmid17603084-
dc.identifier.doi10.1016/j.mvr.2007.04.010-
dc.identifier.urihttp://hdl.handle.net/10033/25714-
dc.description.abstractSurvival of ex vivo constructed tissues after transplantation is limited by insufficient oxygen and nutrient supply. Therefore, strategies aiming at improvement of neovascularization of engineered tissues are a key issue in tissue engineering applications. This in vitro study aimed at exploring the usability of osteogenically differentiated human mesenchymal stem cells (MSCs) as carriers of the angiogenic growth factor genes vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) for therapeutic angiogenesis in bone tissue engineering. The ex vivo adenoviral vector mediated transduction into osteogenically differentiated MSCs revealed a highly efficient and long lasting expression of the transgenes. Biological activity of VEGF and Ang-1 secreted from transduced cells was confirmed by analyzing the sprouting, proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) in response to conditioned medium obtained from transduced cells. The transduced osteogenically differentiated MSCs described in this report may be suitable for inducing neovascularization in bone tissue engineering applications.en
dc.language.isoenen
dc.subject.meshAdenoviridaeen
dc.subject.meshAdult Stem Cellsen
dc.subject.meshAngiopoietin-1en
dc.subject.meshApoptosisen
dc.subject.meshCell Differentiationen
dc.subject.meshCell Proliferationen
dc.subject.meshCells, Cultureden
dc.subject.meshCulture Media, Conditioneden
dc.subject.meshEndothelial Cellsen
dc.subject.meshGenetic Vectorsen
dc.subject.meshHumansen
dc.subject.meshMesenchymal Stem Cellsen
dc.subject.meshNeovascularization, Physiologicen
dc.subject.meshOsteoblastsen
dc.subject.meshOsteogenesisen
dc.subject.meshSpheroids, Cellularen
dc.subject.meshTime Factorsen
dc.subject.meshTissue Engineeringen
dc.subject.meshTransduction, Geneticen
dc.subject.meshVascular Endothelial Growth Factor Aen
dc.titleHigh efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, Germany.en
dc.identifier.journalMicrovascular researchen

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