2.50
Hdl Handle:
http://hdl.handle.net/10033/266513
Title:
Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.
Authors:
Dandekar, Prajakta; Jain, Ratnesh; Keil, Manuel; Loretz, Brigitta; Muijs, Leon; Schneider, Marc ( 0000-0002-9260-7357 ) ; Auerbach, Dagmar; Jung, Gregor; Lehr, Claus-Michael; Wenz, Gerhard
Abstract:
Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.
Affiliation:
Department of Drug Delivery, Helmholtz-Institute for Pharmaceutical Research Saarland, Helmholtz-Center for Infection Research (HZI), Saarland University, D-66123 Saarbrücken, Germany.
Citation:
Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. 2012, 164 (3):387-93 J Control Release
Journal:
Journal of controlled release : official journal of the Controlled Release Society
Issue Date:
28-Dec-2012
URI:
http://hdl.handle.net/10033/266513
DOI:
10.1016/j.jconrel.2012.06.040
PubMed ID:
22789529
Type:
Article
Language:
en
ISSN:
1873-4995
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorDandekar, Prajaktaen_GB
dc.contributor.authorJain, Ratneshen_GB
dc.contributor.authorKeil, Manuelen_GB
dc.contributor.authorLoretz, Brigittaen_GB
dc.contributor.authorMuijs, Leonen_GB
dc.contributor.authorSchneider, Marcen_GB
dc.contributor.authorAuerbach, Dagmaren_GB
dc.contributor.authorJung, Gregoren_GB
dc.contributor.authorLehr, Claus-Michaelen_GB
dc.contributor.authorWenz, Gerharden_GB
dc.date.accessioned2013-01-22T15:26:29Zen
dc.date.available2013-01-22T15:26:29Zen
dc.date.issued2012-12-28en
dc.identifier.citationCellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. 2012, 164 (3):387-93 J Control Releaseen_GB
dc.identifier.issn1873-4995en
dc.identifier.pmid22789529en
dc.identifier.doi10.1016/j.jconrel.2012.06.040en
dc.identifier.urihttp://hdl.handle.net/10033/266513en
dc.description.abstractCationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of controlled release : official journal of the Controlled Release Societyen_GB
dc.titleCellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Drug Delivery, Helmholtz-Institute for Pharmaceutical Research Saarland, Helmholtz-Center for Infection Research (HZI), Saarland University, D-66123 Saarbrücken, Germany.en_GB
dc.identifier.journalJournal of controlled release : official journal of the Controlled Release Societyen_GB

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