Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment.

2.50
Hdl Handle:
http://hdl.handle.net/10033/269913
Title:
Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment.
Authors:
Weingärtner, Adrien; Kemmer, Gerdi; Müller, Frederic D; Zampieri, Ricardo Andrade; Gonzaga dos Santos, Marcos; Schiller, Jürgen; Pomorski, Thomas Günther
Abstract:
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vertebrate host macrophages. A recent model suggests that promastigote and amastigote forms of the parasite mimic mammalian apoptotic cells by exposing phosphatidylserine (PS) at the cell surface to trigger their phagocytic uptake into host macrophages. PS presentation at the cell surface is typically analyzed using fluorescence-labeled annexin V. Here we show that Leishmania promastigotes can be stained by fluorescence-labeled annexin V upon permeabilization or miltefosine treatment. However, combined lipid analysis by thin-layer chromatography, mass spectrometry and (31)P nuclear magnetic resonance (NMR) spectroscopy revealed that Leishmania promastigotes lack any detectable amount of PS. Instead, we identified several other phospholipid classes such phosphatidic acid, phosphatidylethanolamine; phosphatidylglycerol and phosphatidylinositol as candidate lipids enabling annexin V staining.
Affiliation:
Institute of Biology, Humboldt-Universität zu Berlin, Berlin, Germany.
Citation:
Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment. 2012, 7 (8):e42070 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/269913
DOI:
10.1371/journal.pone.0042070
PubMed ID:
22870283
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group immune aging and chronic infections (IMCI)

Full metadata record

DC FieldValue Language
dc.contributor.authorWeingärtner, Adrienen_GB
dc.contributor.authorKemmer, Gerdien_GB
dc.contributor.authorMüller, Frederic Den_GB
dc.contributor.authorZampieri, Ricardo Andradeen_GB
dc.contributor.authorGonzaga dos Santos, Marcosen_GB
dc.contributor.authorSchiller, Jürgenen_GB
dc.contributor.authorPomorski, Thomas Güntheren_GB
dc.date.accessioned2013-02-21T09:09:40Z-
dc.date.available2013-02-21T09:09:40Z-
dc.date.issued2012-
dc.identifier.citationLeishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment. 2012, 7 (8):e42070 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22870283-
dc.identifier.doi10.1371/journal.pone.0042070-
dc.identifier.urihttp://hdl.handle.net/10033/269913-
dc.description.abstractThe protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vertebrate host macrophages. A recent model suggests that promastigote and amastigote forms of the parasite mimic mammalian apoptotic cells by exposing phosphatidylserine (PS) at the cell surface to trigger their phagocytic uptake into host macrophages. PS presentation at the cell surface is typically analyzed using fluorescence-labeled annexin V. Here we show that Leishmania promastigotes can be stained by fluorescence-labeled annexin V upon permeabilization or miltefosine treatment. However, combined lipid analysis by thin-layer chromatography, mass spectrometry and (31)P nuclear magnetic resonance (NMR) spectroscopy revealed that Leishmania promastigotes lack any detectable amount of PS. Instead, we identified several other phospholipid classes such phosphatidic acid, phosphatidylethanolamine; phosphatidylglycerol and phosphatidylinositol as candidate lipids enabling annexin V staining.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAnnexin A5en_GB
dc.subject.meshAntiprotozoal Agentsen_GB
dc.subject.meshCell Membrane Permeabilityen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLeishmaniaen_GB
dc.subject.meshPhagocytosisen_GB
dc.subject.meshPhosphatidylserinesen_GB
dc.subject.meshPhosphorylcholineen_GB
dc.titleLeishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment.en
dc.typeArticleen
dc.contributor.departmentInstitute of Biology, Humboldt-Universität zu Berlin, Berlin, Germany.en_GB
dc.identifier.journalPloS oneen_GB

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