2.50
Hdl Handle:
http://hdl.handle.net/10033/269940
Title:
Metaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation.
Authors:
Guazzaroni, María-Eugenia; Herbst, Florian-Alexander; Lores, Iván; Tamames, Javier; Peláez, Ana Isabel; López-Cortés, Nieves; Alcaide, María; Del Pozo, Mercedes V; Vieites, José María; von Bergen, Martin; Gallego, José Luis R; Bargiela, Rafael; López-López, Arantxa; Pieper, Dietmar H; Rosselló-Móra, Ramón; Sánchez, Jesús; Seifert, Jana; Ferrer, Manuel
Abstract:
Microbial metabolism in aromatic-contaminated environments has important ecological implications, and obtaining a complete understanding of this process remains a relevant goal. To understand the roles of biodiversity and aromatic-mediated genetic and metabolic rearrangements, we conducted 'OMIC' investigations in an anthropogenically influenced and polyaromatic hydrocarbon (PAH)-contaminated soil with (Nbs) or without (N) bio-stimulation with calcium ammonia nitrate, NH(4)NO(3) and KH(2)PO(4) and the commercial surfactant Iveysol, plus two naphthalene-enriched communities derived from both soils (CN2 and CN1, respectively). Using a metagenomic approach, a total of 52, 53, 14 and 12 distinct species (according to operational phylogenetic units (OPU) in our work equivalent to taxonomic species) were identified in the N, Nbs, CN1 and CN2 communities, respectively. Approximately 10 out of 95 distinct species and 238 out of 3293 clusters of orthologous groups (COGs) protein families identified were clearly stimulated under the assayed conditions, whereas only two species and 1465 COGs conformed to the common set in all of the mesocosms. Results indicated distinct biodegradation capabilities for the utilisation of potential growth-supporting aromatics, which results in bio-stimulated communities being extremely fit to naphthalene utilisation and non-stimulated communities exhibiting a greater metabolic window than previously predicted. On the basis of comparing protein expression profiles and metagenome data sets, inter-alia interactions among members were hypothesised. The utilisation of curated databases is discussed and used for first time to reconstruct 'presumptive' degradation networks for complex microbial communities.
Affiliation:
Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.
Citation:
Metaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation. 2013, 7 (1):122-36 ISME J
Journal:
The ISME journal
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10033/269940
DOI:
10.1038/ismej.2012.82
PubMed ID:
22832345
Type:
Article
Language:
en
ISSN:
1751-7370
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorGuazzaroni, María-Eugeniaen_GB
dc.contributor.authorHerbst, Florian-Alexanderen_GB
dc.contributor.authorLores, Ivánen_GB
dc.contributor.authorTamames, Javieren_GB
dc.contributor.authorPeláez, Ana Isabelen_GB
dc.contributor.authorLópez-Cortés, Nievesen_GB
dc.contributor.authorAlcaide, Maríaen_GB
dc.contributor.authorDel Pozo, Mercedes Ven_GB
dc.contributor.authorVieites, José Maríaen_GB
dc.contributor.authorvon Bergen, Martinen_GB
dc.contributor.authorGallego, José Luis Ren_GB
dc.contributor.authorBargiela, Rafaelen_GB
dc.contributor.authorLópez-López, Arantxaen_GB
dc.contributor.authorPieper, Dietmar Hen_GB
dc.contributor.authorRosselló-Móra, Ramónen_GB
dc.contributor.authorSánchez, Jesúsen_GB
dc.contributor.authorSeifert, Janaen_GB
dc.contributor.authorFerrer, Manuelen_GB
dc.date.accessioned2013-02-21T14:02:20Z-
dc.date.available2013-02-21T14:02:20Z-
dc.date.issued2013-01-
dc.identifier.citationMetaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation. 2013, 7 (1):122-36 ISME Jen_GB
dc.identifier.issn1751-7370-
dc.identifier.pmid22832345-
dc.identifier.doi10.1038/ismej.2012.82-
dc.identifier.urihttp://hdl.handle.net/10033/269940-
dc.description.abstractMicrobial metabolism in aromatic-contaminated environments has important ecological implications, and obtaining a complete understanding of this process remains a relevant goal. To understand the roles of biodiversity and aromatic-mediated genetic and metabolic rearrangements, we conducted 'OMIC' investigations in an anthropogenically influenced and polyaromatic hydrocarbon (PAH)-contaminated soil with (Nbs) or without (N) bio-stimulation with calcium ammonia nitrate, NH(4)NO(3) and KH(2)PO(4) and the commercial surfactant Iveysol, plus two naphthalene-enriched communities derived from both soils (CN2 and CN1, respectively). Using a metagenomic approach, a total of 52, 53, 14 and 12 distinct species (according to operational phylogenetic units (OPU) in our work equivalent to taxonomic species) were identified in the N, Nbs, CN1 and CN2 communities, respectively. Approximately 10 out of 95 distinct species and 238 out of 3293 clusters of orthologous groups (COGs) protein families identified were clearly stimulated under the assayed conditions, whereas only two species and 1465 COGs conformed to the common set in all of the mesocosms. Results indicated distinct biodegradation capabilities for the utilisation of potential growth-supporting aromatics, which results in bio-stimulated communities being extremely fit to naphthalene utilisation and non-stimulated communities exhibiting a greater metabolic window than previously predicted. On the basis of comparing protein expression profiles and metagenome data sets, inter-alia interactions among members were hypothesised. The utilisation of curated databases is discussed and used for first time to reconstruct 'presumptive' degradation networks for complex microbial communities.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The ISME journalen_GB
dc.titleMetaproteogenomic insights beyond bacterial response to naphthalene exposure and bio-stimulation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.en_GB
dc.identifier.journalThe ISME journalen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.