Subcellular localization and function of an epitope-tagged p7 viroporin in hepatitis C virus-producing cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/270512
Title:
Subcellular localization and function of an epitope-tagged p7 viroporin in hepatitis C virus-producing cells.
Authors:
Vieyres, Gabrielle; Brohm, Christiane; Friesland, Martina; Gentzsch, Juliane; Wölk, Benno; Roingeard, Philippe; Steinmann, Eike; Pietschmann, Thomas
Abstract:
The hepatitis C virus (HCV) viroporin p7 is crucial for production of infectious viral progeny. However, its role in the viral replication cycle remains incompletely understood, in part due to the poor availability of p7-specific antibodies. To circumvent this obstacle, we inserted two consecutive hemagglutinin (HA) epitope tags at its N terminus. HA-tagged p7 reduced peak virus titers ca. 10-fold and decreased kinetics of virus production compared to the wild-type virus. However, HA-tagged p7 rescued virus production of a mutant virus lacking p7, thus providing formal proof that the tag does not disrupt p7 function. In HCV-producing cells, p7 displayed a reticular staining pattern which colocalized with the HCV envelope glycoprotein 2 (E2) but also partially with viral nonstructural proteins 2, 3, and 5A. Using coimmunoprecipitation, we confirmed a specific interaction between p7 and NS2, whereas we did not detect a stable interaction with core, E2, or NS5A. Moreover, we did not observe p7 incorporation into affinity-purified virus particles. Consistently, there was no evidence supporting a role of p7 in viral entry, as an anti-HA antibody was not able to neutralize Jc1 virus produced from an HA-p7-tagged genome. Collectively, these findings highlight a stable interaction between p7 and NS2 which is likely crucial for production of infectious HCV particles. Use of this functional epitope-tagged p7 variant should facilitate the analysis of the final steps of the HCV replication cycle.
Affiliation:
Institute of Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, Hannover, Germany.
Citation:
Subcellular localization and function of an epitope-tagged p7 viroporin in hepatitis C virus-producing cells. 2013, 87 (3):1664-78 J. Virol.
Journal:
Journal of virology
Issue Date:
Feb-2013
URI:
http://hdl.handle.net/10033/270512
DOI:
10.1128/JVI.02782-12
PubMed ID:
23175364
Type:
Article
Language:
en
ISSN:
1098-5514
Appears in Collections:
publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorVieyres, Gabrielleen_GB
dc.contributor.authorBrohm, Christianeen_GB
dc.contributor.authorFriesland, Martinaen_GB
dc.contributor.authorGentzsch, Julianeen_GB
dc.contributor.authorWölk, Bennoen_GB
dc.contributor.authorRoingeard, Philippeen_GB
dc.contributor.authorSteinmann, Eikeen_GB
dc.contributor.authorPietschmann, Thomasen_GB
dc.date.accessioned2013-02-26T15:43:22Zen
dc.date.available2013-02-26T15:43:22Zen
dc.date.issued2013-02en
dc.identifier.citationSubcellular localization and function of an epitope-tagged p7 viroporin in hepatitis C virus-producing cells. 2013, 87 (3):1664-78 J. Virol.en_GB
dc.identifier.issn1098-5514en
dc.identifier.pmid23175364en
dc.identifier.doi10.1128/JVI.02782-12en
dc.identifier.urihttp://hdl.handle.net/10033/270512en
dc.description.abstractThe hepatitis C virus (HCV) viroporin p7 is crucial for production of infectious viral progeny. However, its role in the viral replication cycle remains incompletely understood, in part due to the poor availability of p7-specific antibodies. To circumvent this obstacle, we inserted two consecutive hemagglutinin (HA) epitope tags at its N terminus. HA-tagged p7 reduced peak virus titers ca. 10-fold and decreased kinetics of virus production compared to the wild-type virus. However, HA-tagged p7 rescued virus production of a mutant virus lacking p7, thus providing formal proof that the tag does not disrupt p7 function. In HCV-producing cells, p7 displayed a reticular staining pattern which colocalized with the HCV envelope glycoprotein 2 (E2) but also partially with viral nonstructural proteins 2, 3, and 5A. Using coimmunoprecipitation, we confirmed a specific interaction between p7 and NS2, whereas we did not detect a stable interaction with core, E2, or NS5A. Moreover, we did not observe p7 incorporation into affinity-purified virus particles. Consistently, there was no evidence supporting a role of p7 in viral entry, as an anti-HA antibody was not able to neutralize Jc1 virus produced from an HA-p7-tagged genome. Collectively, these findings highlight a stable interaction between p7 and NS2 which is likely crucial for production of infectious HCV particles. Use of this functional epitope-tagged p7 variant should facilitate the analysis of the final steps of the HCV replication cycle.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of virologyen_GB
dc.titleSubcellular localization and function of an epitope-tagged p7 viroporin in hepatitis C virus-producing cells.en
dc.typeArticleen
dc.contributor.departmentInstitute of Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, Hannover, Germany.en_GB
dc.identifier.journalJournal of virologyen_GB

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